PMID- 10606742
OWN - NLM
STAT- MEDLINE
DCOM- 20000119
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 463
IP  - 3
DP  - 1999 Dec 17
TI  - Cloning, functional characterisation and population analysis of a variant form of
      the human glycine type 2 transporter.
PG  - 301-6
AB  - Two forms of glycine transporter have been described to date, GlyT-1 and GlyT-2. 
      The GlyT-2 form is expressed mainly in the spinal cord, brainstem and cerebellum.
      Here we describe the identification of a variant form of the human GlyT-2 (SC6), 
      showing three amino acid changes to the previously reported protein. Population
      analysis identified the allele causing one of the polymorphisms, D463N, at 10%
      within the population with 3% being homozygous for the change. We also
      transfected our new variant into mammalian cells and compared it to the published
      cDNA, showing that the three amino acid changes present have no major effect on
      the biochemical properties of the transporter.
FAU - Evans, J
AU  - Evans J
AD  - Molecular Biology Department, SmithKline Beecham Pharmaceuticals, Harlow, UK.
FAU - Herdon, H
AU  - Herdon H
FAU - Cairns, W
AU  - Cairns W
FAU - O'Brien, E
AU  - O'Brien E
FAU - Chapman, C
AU  - Chapman C
FAU - Terrett, J
AU  - Terrett J
FAU - Gloger, I
AU  - Gloger I
LA  - eng
PT  - Journal Article
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Amino Acid Transport Systems, Neutral)
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Glycine Plasma Membrane Transport Proteins)
RN  - 0 (SLC6A5 protein, human)
RN  - 0 (SLC6A9 protein, human)
RN  - TE7660XO1C (Glycine)
SB  - IM
MH  - Amino Acid Sequence
MH  - *Amino Acid Transport Systems, Neutral
MH  - Carrier Proteins/chemistry/*genetics/metabolism
MH  - Cloning, Molecular
MH  - DNA, Complementary/chemistry
MH  - Gene Frequency
MH  - Genotype
MH  - Glycine/*metabolism
MH  - Glycine Plasma Membrane Transport Proteins
MH  - Humans
MH  - Molecular Sequence Data
MH  - Mutagenesis, Site-Directed
MH  - Polymorphism, Genetic
MH  - Sequence Alignment
MH  - Spinal Cord/metabolism
MH  - Transfection
EDAT- 1999/12/22 00:00
MHDA- 1999/12/22 00:01
CRDT- 1999/12/22 00:00
PHST- 1999/12/22 00:00 [pubmed]
PHST- 1999/12/22 00:01 [medline]
PHST- 1999/12/22 00:00 [entrez]
AID - S0014-5793(99)01636-1 [pii]
AID - 10.1016/s0014-5793(99)01636-1 [doi]
PST - ppublish
SO  - FEBS Lett. 1999 Dec 17;463(3):301-6. doi: 10.1016/s0014-5793(99)01636-1.