PMID- 10606657
OWN - NLM
STAT- MEDLINE
DCOM- 20000313
LR  - 20190501
IS  - 1362-4962 (Electronic)
IS  - 0305-1048 (Linking)
VI  - 28
IP  - 2
DP  - 2000 Jan 15
TI  - The dhfr oribeta-binding protein RIP60 contains 15 zinc fingers: DNA binding and 
      looping by the central three fingers and an associated proline-rich region.
PG  - 570-81
AB  - Initiation of DNA replication occurs with high frequency within oribeta, a short 
      region 3' to the Chinese hamster dhfr gene. Homodimers of RIP60 (replication
      initiation-region protein 60 kDA) purified from nuclear extract bind two ATT-rich
      sites in oribeta and foster the formation of a twisted 720 bp DNA loop in vitro. 
      Using a one hybrid screen in yeast, we have cloned the cDNA for human RIP60.
      RIP60 contains 15 C(2)H(2)zinc finger (ZF) DNA binding motifs organized in three 
      clusters, termed hand Z1 (ZFs 1-5), hand Z2 (ZFs 6-8) and hand Z3 (ZFs 9-15). A
      proline-rich region is located between hands Z2 and Z3. Gel mobility shift and
      DNase I footprinting experiments show hands Z1 and Z2 independently bind the
      oribeta RIP60 sites specifically, but with different affinities. Hand Z3 binds
      DNA, but displays no specificity for RIP60 sites. Ligation enhancement, DNase I
      footprinting, and atomic force microscopy assays show that hand Z2 and a portion 
      of the associated proline-rich region is sufficient for protein multimerization
      on DNA and DNA looping in vitro. Polyomavirus origin-dependent plasmid
      replication assays show RIP60 has weak replication enhancer activity, suggesting 
      that RIP60 does not harbor a transcriptional transactivation domain. Because
      vertebrate origins of replication have no known consensus sequence, we suggest
      that sequence-specific DNA binding proteins such as RIP60 may act as accessory
      factors in origin identification prior to the assembly of pre-initiation
      complexes.
FAU - Houchens, C R
AU  - Houchens CR
AD  - Department of Pathology, University of Vermont, Burlington, VT 05405, USA.
FAU - Montigny, W
AU  - Montigny W
FAU - Zeltser, L
AU  - Zeltser L
FAU - Dailey, L
AU  - Dailey L
FAU - Gilbert, J M
AU  - Gilbert JM
FAU - Heintz, N H
AU  - Heintz NH
LA  - eng
SI  - GENBANK/AF201303
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (Basic Helix-Loop-Helix Leucine Zipper Transcription Factors)
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (REPIN1 protein, human)
RN  - 9007-49-2 (DNA)
RN  - 9DLQ4CIU6V (Proline)
RN  - EC 1.5.1.3 (Tetrahydrofolate Dehydrogenase)
RN  - EC 2.5.1.18 (Glutathione Transferase)
SB  - IM
MH  - 3T3 Cells
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
MH  - Cloning, Molecular
MH  - DNA/chemistry/*metabolism
MH  - DNA Footprinting
MH  - DNA Primers
MH  - DNA, Complementary
MH  - DNA-Binding Proteins/chemistry/genetics/*metabolism
MH  - Glutathione Transferase/metabolism
MH  - Humans
MH  - Mice
MH  - Microscopy, Atomic Force
MH  - Molecular Sequence Data
MH  - *Nucleic Acid Conformation
MH  - Polyomavirus/genetics
MH  - Proline/*metabolism
MH  - Protein Binding
MH  - Replication Origin
MH  - Tetrahydrofolate Dehydrogenase/*genetics
MH  - *Zinc Fingers
PMC - PMC102514
EDAT- 1999/12/22 00:00
MHDA- 2000/03/18 00:00
CRDT- 1999/12/22 00:00
PHST- 1999/12/22 00:00 [pubmed]
PHST- 2000/03/18 00:00 [medline]
PHST- 1999/12/22 00:00 [entrez]
AID - gkd141 [pii]
AID - 10.1093/nar/28.2.570 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 2000 Jan 15;28(2):570-81. doi: 10.1093/nar/28.2.570.