PMID- 10606637
OWN - NLM
STAT- MEDLINE
DCOM- 20000313
LR  - 20190501
IS  - 1362-4962 (Electronic)
IS  - 0305-1048 (Linking)
VI  - 28
IP  - 2
DP  - 2000 Jan 15
TI  - Members of the SRY family regulate the human LINE retrotransposons.
PG  - 411-5
AB  - LINEs are endogenous mobile genetic elements which have dispersed and accumulated
      in the genomes of most higher eukaryotes via germline transposition, with up to
      100 000 copies for the human LINE-1 (L1H) sequences. Although severely repressed 
      in most normal tissues, L1H is still functional, with evidence for both germline 
      and somatic-essentially in tumors-transpositions. Yet, no transcription factor
      that could regulate their transcription and be responsible for their
      transposition has hitherto been described. Here we show that factors belonging to
      the family of the testis-determining factor gene SRY (the SOX family) can
      modulate L1H promoter activity over a 10-fold range in a transient transfection
      assay using a luciferase reporter gene. These effects depend on two functional
      SRY binding sites which can be identified within the L1H promoter via mobility
      shift assays. Induction of endogenous L1Hs upon ectopic expression of the SOX11
      transcription factor is further demonstrated, thus strengthening the
      physiological relevance of these new-and highly dispersed-target sites for the
      otherwise unclassical transcription factors of the SRY family.
FAU - Tchenio, T
AU  - Tchenio T
AD  - Unite des Retrovirus Endogenes et Elements Retroi des des Eucaryotes Superieurs, 
      UMR1573 CNRS, Institut Gustave Roussy, 39 rue Camille Desmoulins, 94805 Villejuif
      Cedex, France.
FAU - Casella, J F
AU  - Casella JF
FAU - Heidmann, T
AU  - Heidmann T
LA  - eng
PT  - Journal Article
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (SRY protein, human)
RN  - 0 (Sex-Determining Region Y Protein)
RN  - 0 (Transcription Factors)
RN  - 9007-49-2 (DNA)
RN  - EC 1.13.12.- (Luciferases)
SB  - IM
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Cell Line
MH  - DNA
MH  - DNA-Binding Proteins/*physiology
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Gene Expression Regulation/*physiology
MH  - Genes, Reporter
MH  - Humans
MH  - *Long Interspersed Nucleotide Elements
MH  - Luciferases/genetics
MH  - *Nuclear Proteins
MH  - Promoter Regions, Genetic
MH  - Sex-Determining Region Y Protein
MH  - *Transcription Factors
PMC - PMC102531
EDAT- 1999/12/22 00:00
MHDA- 2000/03/18 00:00
CRDT- 1999/12/22 00:00
PHST- 1999/12/22 00:00 [pubmed]
PHST- 2000/03/18 00:00 [medline]
PHST- 1999/12/22 00:00 [entrez]
AID - gkd159 [pii]
AID - 10.1093/nar/28.2.411 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 2000 Jan 15;28(2):411-5. doi: 10.1093/nar/28.2.411.