PMID- 10606267
OWN - NLM
STAT- MEDLINE
DCOM- 20000111
LR  - 20190607
IS  - 1355-8382 (Print)
IS  - 1355-8382 (Linking)
VI  - 5
IP  - 12
DP  - 1999 Dec
TI  - Identification of a protein component of a mammalian tRNA(Sec) complex implicated
      in the decoding of UGA as selenocysteine.
PG  - 1561-9
AB  - This report describes a novel RNA-binding protein, SECp43, that associates
      specifically with mammalian selenocysteine tRNA (tRNA(Sec)). SECp43, identified
      from a degenerate PCR screen, is a highly conserved protein with two
      ribonucleoprotein-binding domains and a polar/acidic carboxy terminus. The
      protein and corresponding mRNA are generally expressed in rat tissues and
      mammalian cell lines. To gain insight into the biological role of SECp43,
      affinity-purified antibody was employed to identify its molecular partners.
      Surprisingly, the application of native HeLa cell extracts to a SECp43 antibody
      column results in the purification of a 90-nt RNA species identified by direct
      sequencing and Northern blot analysis as tRNA(Sec). The purification of tRNA(Sec)
      by the antibody column is striking, based on the low abundance of this tRNA
      species. Using recombinant SECp43 as a probe for interacting protein partners, we
      also identify a 48-kDa interacting protein, which is a possible component of the 
      mammalian selenocysteine insertion (SECIS) pathway. To our knowledge, SECp43 is
      the first cloned protein demonstrated to associate specifically with eukaryotic
      tRNA(Sec).
FAU - Ding, F
AU  - Ding F
AD  - Department of Biological Sciences, University of Pittsburgh, Pennsylvania 15260, 
      USA.
FAU - Grabowski, P J
AU  - Grabowski PJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - RNA
JT  - RNA (New York, N.Y.)
JID - 9509184
RN  - 0 (Codon)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Transfer, Amino Acid-Specific)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Secp43 protein, rat)
RN  - 0 (tRNA, selenocysteine-)
RN  - 0CH9049VIS (Selenocysteine)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cell Line
MH  - Codon/*genetics
MH  - HeLa Cells
MH  - Humans
MH  - Mammals
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Nucleic Acid Conformation
MH  - RNA, Messenger/genetics
MH  - RNA, Transfer, Amino Acid-Specific/*genetics/isolation & purification/metabolism
MH  - RNA-Binding Proteins/*genetics/*metabolism
MH  - Rats
MH  - Reverse Transcriptase Polymerase Chain Reaction/methods
MH  - Selenocysteine/*genetics
MH  - Sequence Alignment
MH  - Sequence Homology, Nucleic Acid
PMC - PMC1369878
EDAT- 1999/12/22 00:00
MHDA- 1999/12/22 00:01
CRDT- 1999/12/22 00:00
PHST- 1999/12/22 00:00 [pubmed]
PHST- 1999/12/22 00:01 [medline]
PHST- 1999/12/22 00:00 [entrez]
AID - 10.1017/s1355838299991598 [doi]
PST - ppublish
SO  - RNA. 1999 Dec;5(12):1561-9. doi: 10.1017/s1355838299991598.