PMID- 10606266
OWN - NLM
STAT- MEDLINE
DCOM- 20000111
LR  - 20190607
IS  - 1355-8382 (Print)
IS  - 1355-8382 (Linking)
VI  - 5
IP  - 12
DP  - 1999 Dec
TI  - PUF60: a novel U2AF65-related splicing activity.
PG  - 1548-60
AB  - We have identified a new pyrimidine-tract binding factor, PUF, that is required, 
      together with U2AF, for efficient reconstitution of RNA splicing in vitro. The
      activity has been purified and consists of two proteins, PUF60 and the previously
      described splicing factor p54. p54 and PUF60 form a stable complex in vitro when 
      cotranslated in a reaction mixture. PUF activity, in conjunction with U2AF,
      facilitates the association of U2 snRNP with the pre-mRNA. This reaction is
      dependent upon the presence of the large subunit of U2AF, U2AF65, but not the
      small subunit U2AF35. PUF60 is homologous to both U2AF65 and the yeast splicing
      factor Mud2p. The C-terminal domain of PUF60, the PUMP domain, is distantly
      related to the RNA-recognition motif domain, and is probably important in
      protein-protein interactions.
FAU - Page-McCaw, P S
AU  - Page-McCaw PS
AD  - Center for Cancer Research and Department of Biology, Massachusetts Institute of 
      Technology, Cambridge 02139, USA.
FAU - Amonlirdviman, K
AU  - Amonlirdviman K
FAU - Sharp, P A
AU  - Sharp PA
LA  - eng
GR  - P30-CA14051/CA/NCI NIH HHS/United States
GR  - R01-AI32486/AI/NIAID NIH HHS/United States
GR  - R37-GM34277/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - RNA
JT  - RNA (New York, N.Y.)
JID - 9509184
RN  - 0 (NM23 Nucleoside Diphosphate Kinases)
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Ribonucleoproteins)
RN  - 0 (Sfrs11 protein)
RN  - 0 (Splicing Factor U2AF)
RN  - 0 (Transcription Factors)
RN  - 0 (U2AF2 protein, human)
RN  - 170974-22-8 (Serine-Arginine Splicing Factors)
RN  - 27416-86-0 (Poly U)
RN  - EC 2.7.4.6 (Nucleoside-Diphosphate Kinase)
RN  - EC 3.6.5.2 (Monomeric GTP-Binding Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Cell Nucleus/metabolism
MH  - Chromatography, Affinity
MH  - HeLa Cells
MH  - Humans
MH  - Molecular Sequence Data
MH  - *Monomeric GTP-Binding Proteins
MH  - NM23 Nucleoside Diphosphate Kinases
MH  - Nuclear Proteins/metabolism
MH  - *Nucleoside-Diphosphate Kinase
MH  - Poly U
MH  - *RNA Splicing
MH  - RNA-Binding Proteins/chemistry/*metabolism
MH  - Ribonucleoproteins/*metabolism
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Serine-Arginine Splicing Factors
MH  - Splicing Factor U2AF
MH  - Transcription Factors/chemistry/isolation & purification/*metabolism
PMC - PMC1369877
EDAT- 1999/12/22 00:00
MHDA- 1999/12/22 00:01
CRDT- 1999/12/22 00:00
PHST- 1999/12/22 00:00 [pubmed]
PHST- 1999/12/22 00:01 [medline]
PHST- 1999/12/22 00:00 [entrez]
AID - 10.1017/s1355838299991938 [doi]
PST - ppublish
SO  - RNA. 1999 Dec;5(12):1548-60. doi: 10.1017/s1355838299991938.