PMID- 10605026
OWN - NLM
STAT- MEDLINE
DCOM- 20000119
LR  - 20220318
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 164
IP  - 1
DP  - 2000 Jan 1
TI  - HLA-F is a predominantly empty, intracellular, TAP-associated MHC class Ib
      protein with a restricted expression pattern.
PG  - 319-28
AB  - HLA-F is currently the most enigmatic of the human MHC-encoded class Ib genes. We
      have investigated the expression of HLA-F using a specific Ab raised against a
      synthetic peptide corresponding to amino acids 61-84 in the alpha1 domain of the 
      predicted HLA-F protein. HLA-F is expressed as a beta2-microglobulin-associated, 
      42-kDa protein that shows a restricted tissue distribution. To date, we have
      detected this product only in peripheral blood B cells, B cell lines, and tissues
      containing B cells, in particular adult tonsil and fetal liver, a major site of B
      cell development. Thermostability assays suggest that HLA-F is expressed as an
      empty heterodimer devoid of peptide. Consistent with this, studies using
      endoglycosidase-H and cell surface immunoprecipitations also indicate that the
      overwhelming majority of HLA-F contains an immature oligosaccharide component and
      is expressed inside the cell. We have found that IFN-gamma treatment induces
      expression of HLA-F mRNA and HLA-F protein, but that this does not result in
      concomitant cell surface expression. HLA-F associates with at least two
      components of the conventional class I assembly pathway, calreticulin and TAP.
      The unusual characteristics of the predicted peptide-binding groove together with
      the predominantly intracellular localization raise the possibility that HLA-F may
      be capable of binding only a restricted set of peptides.
FAU - Wainwright, S D
AU  - Wainwright SD
AD  - Department of Clinical Medicine, Division of Obstetrics and Gynaecology,
      University of Bristol, St. Michael's Hospital, United Kingdom.
FAU - Biro, P A
AU  - Biro PA
FAU - Holmes, C H
AU  - Holmes CH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (ATP Binding Cassette Transporter, Subfamily B, Member 2)
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-F antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Peptides)
RN  - 0 (TAP1 protein, human)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - ATP Binding Cassette Transporter, Subfamily B, Member 2
MH  - ATP-Binding Cassette Transporters/*metabolism
MH  - Adult
MH  - Amino Acid Sequence
MH  - Antigen Presentation
MH  - Cell Line
MH  - Gene Expression Regulation/immunology
MH  - HLA Antigens/*biosynthesis/genetics/*isolation & purification/metabolism
MH  - Histocompatibility Antigens Class I/*biosynthesis/genetics/*isolation &
      purification/metabolism
MH  - Humans
MH  - Interferon-gamma/pharmacology
MH  - Intracellular Fluid/*immunology/*metabolism
MH  - Jurkat Cells
MH  - Molecular Sequence Data
MH  - Organ Specificity/immunology
MH  - Peptides/immunology/metabolism
MH  - Protein Binding/immunology
MH  - Tumor Cells, Cultured
EDAT- 1999/12/22 00:00
MHDA- 1999/12/22 00:01
CRDT- 1999/12/22 00:00
PHST- 1999/12/22 00:00 [pubmed]
PHST- 1999/12/22 00:01 [medline]
PHST- 1999/12/22 00:00 [entrez]
AID - ji_v164n1p319 [pii]
AID - 10.4049/jimmunol.164.1.319 [doi]
PST - ppublish
SO  - J Immunol. 2000 Jan 1;164(1):319-28. doi: 10.4049/jimmunol.164.1.319.