PMID- 10602986
OWN - NLM
STAT- MEDLINE
DCOM- 20000203
LR  - 20190905
IS  - 0938-8990 (Print)
IS  - 0938-8990 (Linking)
VI  - 11
IP  - 1
DP  - 2000 Jan
TI  - An axonemal dynein at the Hybrid Sterility 6 locus: implications for t
      haplotype-specific male sterility and the evolution of species barriers.
PG  - 8-15
AB  - Poor sperm motility characterized by a distinct aberration in flagellar waveform 
      known as "curlicue" is a hallmark of t haplotype (t) homozygous male sterility.
      Previous studies have localized "curlicue" and a flagellar developmental defect, 
      "whipless", to the Hybrid Sterility 6 locus (Hst6), between the markers Pim1 and 
      Crya1. More recent heterospecific breeding experiments between Mus spretus
      (Spretus) and Mus musculus domesticus (Domesticus) have mapped the primary
      source(s) of both "curlicue" and "whipless" to a small sub-locus of Hst6,
      Curlicue a (Ccua). Here we report the complete physical isolation of the Ccua
      locus and the identification of a candidate gene for expression of both
      "whipless" and "curlicue" at its proximal end, an axonemal dynein heavy chain
      gene, Dnahc8, formerly mapped by interspecific backcross analysis near Pim1.
      Dnahc8 mRNA expression commences in the Domesticus wild-type testis just prior to
      flagellar assembly and is testis-specific in the adult male. However, expression 
      of Dnahc8 is not readily evident in the testis of either Spretus or "whipless"
      animals (Domesticus males homozygous for the Spretus allele of Dnahc8). Our
      results argue that Dnahc8 is fundamental to flagellar organization and function
      in Domesticus, but not Spretus, and suggest that Dnahc8 is integral to both Hst6-
      and t-specific male infertility.
FAU - Fossella, J
AU  - Fossella J
AD  - Department of Molecular Biology, Princeton University, Princeton, New Jersey
      08540, USA.
FAU - Samant, S A
AU  - Samant SA
FAU - Silver, L M
AU  - Silver LM
FAU - King, S M
AU  - King SM
FAU - Vaughan, K T
AU  - Vaughan KT
FAU - Olds-Clarke, P
AU  - Olds-Clarke P
FAU - Johnson, K A
AU  - Johnson KA
FAU - Mikami, A
AU  - Mikami A
FAU - Vallee, R B
AU  - Vallee RB
FAU - Pilder, S H
AU  - Pilder SH
LA  - eng
GR  - HD31164/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mamm Genome
JT  - Mammalian genome : official journal of the International Mammalian Genome Society
JID - 9100916
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
RN  - 63231-63-0 (RNA)
RN  - EC 3.6.4.2 (Dyneins)
SB  - IM
EIN - Mamm Genome 2000 Mar;11(3):250
MH  - Animals
MH  - Axons
MH  - Blotting, Northern
MH  - Blotting, Southern
MH  - Chromosome Mapping
MH  - Crosses, Genetic
MH  - DNA Primers/chemistry
MH  - DNA, Complementary/chemistry
MH  - Dyneins/*genetics
MH  - Electrophoresis, Agar Gel
MH  - Female
MH  - Haplotypes
MH  - Infertility, Male/*genetics
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - RNA/chemistry/isolation & purification
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sperm Motility/*genetics
EDAT- 1999/12/22 00:00
MHDA- 1999/12/22 00:01
CRDT- 1999/12/22 00:00
PHST- 1999/12/22 00:00 [pubmed]
PHST- 1999/12/22 00:01 [medline]
PHST- 1999/12/22 00:00 [entrez]
AID - MG99-709 [pii]
AID - 10.1007/s003350010003 [doi]
PST - ppublish
SO  - Mamm Genome. 2000 Jan;11(1):8-15. doi: 10.1007/s003350010003.