PMID- 10602480
OWN - NLM
STAT- MEDLINE
DCOM- 20000106
LR  - 20131121
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 18
IP  - 51
DP  - 1999 Dec 2
TI  - Interaction partners of Dlk/ZIP kinase: co-expression of Dlk/ZIP kinase and Par-4
      results in cytoplasmic retention and apoptosis.
PG  - 7265-73
AB  - Dlk/ZIP kinase is a newly discovered serine/threonine kinase which, due to its
      homology to DAP kinase, was named DAP like kinase, Dlk. This kinase is tightly
      associated with nuclear structures, it undergoes extensive autophosphorylation
      and phosphorylates myosin light chain and core histones H3, H2A and H4 in vitro. 
      Moreover, it possesses a leucine zipper which mediates interaction with
      transcription factor ATF-4, therefore it was called ZIP kinase. We employed the
      yeast two-hybrid system to identify interaction partners of Dlk that might serve 
      as regulators or targets. Besides ATF-4 and others we found Par-4, a modulator of
      transcription factor WT1 and mediator of apoptosis. Complex formation between Dlk
      and Par-4 was confirmed by GST pull-down experiments and kinase reactions in
      vitro and coexpression experiments in vivo. The interaction domain within Dlk was
      mapped to an arginine-rich region between residues 338 - 417, rather than to the 
      leucine zipper. Strikingly, coexpression of Dlk and Par-4 lead to relocation of
      Dlk from the nucleus to the cytoplasm, particularly to actin filaments. These
      interactions provoked a dramatic reorganization of the cytoskeleton and
      morphological symptoms of apoptosis, thus suggesting a functional relationship
      between Dlk and Par-4 in the control of apoptosis.
FAU - Page, G
AU  - Page G
AD  - Institute of Genetics, University of Bonn, Roemerstr. 164, D-53117 Bonn, Germany.
FAU - Kogel, D
AU  - Kogel D
FAU - Rangnekar, V
AU  - Rangnekar V
FAU - Scheidtmann, K H
AU  - Scheidtmann KH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (Carrier Proteins)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Receptors, Fibroblast Growth Factor)
RN  - 0 (prostate apoptosis response-4 protein)
RN  - EC 2.7.11.1 (Death-Associated Protein Kinases)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
RN  - EC 2.7.11.25 (MAP Kinase Kinase Kinases)
RN  - EC 2.7.11.25 (mitogen-activated protein kinase kinase kinase 12)
SB  - IM
MH  - Animals
MH  - Apoptosis/*physiology
MH  - Apoptosis Regulatory Proteins
MH  - Calcium-Calmodulin-Dependent Protein Kinases
MH  - Carrier Proteins/genetics/*metabolism
MH  - Cell Line, Transformed
MH  - Cytoplasm/physiology
MH  - Death-Associated Protein Kinases
MH  - Escherichia coli
MH  - *Gene Expression Regulation
MH  - *Intracellular Signaling Peptides and Proteins
MH  - Leucine Zippers
MH  - MAP Kinase Kinase Kinases/genetics/*metabolism
MH  - Phosphorylation
MH  - Rats
MH  - Receptors, Fibroblast Growth Factor/genetics/metabolism
MH  - Signal Transduction
EDAT- 1999/12/22 00:00
MHDA- 1999/12/22 00:01
CRDT- 1999/12/22 00:00
PHST- 1999/12/22 00:00 [pubmed]
PHST- 1999/12/22 00:01 [medline]
PHST- 1999/12/22 00:00 [entrez]
AID - 10.1038/sj.onc.1203170 [doi]
PST - ppublish
SO  - Oncogene. 1999 Dec 2;18(51):7265-73. doi: 10.1038/sj.onc.1203170.