PMID- 10602013
OWN - NLM
STAT- MEDLINE
DCOM- 20000104
LR  - 20180420
IS  - 0014-2980 (Print)
IS  - 0014-2980 (Linking)
VI  - 29
IP  - 12
DP  - 1999 Dec
TI  - A ubiquitin-like protein which is synergistically inducible by interferon-gamma
      and tumor necrosis factor-alpha.
PG  - 4030-6
AB  - A means of regulating the fate of intracellular proteins is their covalent
      conjugation to ubiquitin-like proteins. A recently discovered ubiquitin-like
      protein is called "diubiquitin" because it consists of two ubiquitin-like domains
      in head-to-tail arrangement. Human diubiquitin is encoded at the telomeric end of
      the MHC class I locus and was previously found to be expressed in dendritic cells
      and mature B cells. We have extended the expression analysis of diubiquitin by
      reverse transcriptase-PCR and Northern blotting in primary endothelial cells and 
      human cancer cell lines derived from nine different tissues. Diubiquitin
      expression was found to be generally and synergistically inducible with the
      cytokines IFN-gamma and TNF-alpha but not with IFN-alpha. Diubiquitin mRNA
      expression was induced within 2 h after cytokine stimulation and was independent 
      of protein neosynthesis but dependent on proteasome activity. The mouse homologue
      of diubiquitin which is also encoded in the MHC class I locus was likewise
      induced with IFN-gamma and TNF-alpha. A general and synergistic induction with
      IFN-gamma and TNF-alpha suggests that diubiquitin may exert its functions in
      antigen presentation or other cellular processes controlled by these two
      cytokines.
FAU - Raasi, S
AU  - Raasi S
AD  - Research Department, Cantonal Hospital St. Gall, St. Gallen, Switzerland.
FAU - Schmidtke, G
AU  - Schmidtke G
FAU - de Giuli, R
AU  - de Giuli R
FAU - Groettrup, M
AU  - Groettrup M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Eur J Immunol
JT  - European journal of immunology
JID - 1273201
RN  - 0 (Antiviral Agents)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Ubiquitins)
RN  - 0 (diubiquitin conjugate)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antiviral Agents/*pharmacology
MH  - Drug Synergism
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - Interferon-gamma/*pharmacology
MH  - Mice
MH  - Molecular Sequence Data
MH  - RNA, Messenger/analysis/biosynthesis
MH  - Sequence Alignment
MH  - Tumor Cells, Cultured
MH  - Tumor Necrosis Factor-alpha/*pharmacology
MH  - Ubiquitins/*biosynthesis/genetics
EDAT- 1999/12/22 00:00
MHDA- 1999/12/22 00:01
CRDT- 1999/12/22 00:00
PHST- 1999/12/22 00:00 [pubmed]
PHST- 1999/12/22 00:01 [medline]
PHST- 1999/12/22 00:00 [entrez]
AID - 10.1002/(SICI)1521-4141(199912)29:12<4030::AID-IMMU4030>3.0.CO;2-Y [doi]
PST - ppublish
SO  - Eur J Immunol. 1999 Dec;29(12):4030-6. doi:
      10.1002/(SICI)1521-4141(199912)29:12<4030::AID-IMMU4030>3.0.CO;2-Y.