PMID- 10602003
OWN - NLM
STAT- MEDLINE
DCOM- 20000104
LR  - 20180420
IS  - 0014-2980 (Print)
IS  - 0014-2980 (Linking)
VI  - 29
IP  - 12
DP  - 1999 Dec
TI  - CD45-associated protein is not essential for the regulation of antigen
      receptor-mediated signal transduction.
PG  - 3951-5
AB  - CD45 is a transmembrane protein tyrosine phosphatase required for signaling
      through the T-and B-cell antigen receptors. In lymphocytes, CD45 interacts with
      CD45-associated protein (CD45AP), a 32 000 Mr phosphoprotein, through their
      respective transmembrane domains. To determine whether CD45AP affects the ability
      of CD45 to regulate antigen receptor signaling, CD45AP-deficient mice were
      generated. Thymocyte development was grossly normal. Moreover, the cellularity of
      the thymus and spleens were normal. CD45 expression on thymocytes and
      splenocytes, ascertained by flow cytometry, was comparable between
      CD45AP-deficient mice and littermate controls. In contrast to a previous report
      (Matsuda et al., J. Exp. Med. 1998 187: 1863 - 1870). CD45AP-deficient and normal
      thymocytes and splenocytes proliferated similarly in response to various mitogens
      or antigen receptor cross-linking. Furthermore, thymocyte CD45-associated
      p56(lck) kinase activity was similar between CD45AP-deficient and normal cells.
      We conclude that CD45AP is not essential for the regulation of Src-family kinase 
      activity by CD45.
FAU - Kung, C
AU  - Kung C
AD  - Howard Hughes Medical Institute, Department of Pathology and Molecular
      Microbiology, Washington University School of Medicine, St. Louis, MO 63110, USA.
FAU - Okumura, M
AU  - Okumura M
FAU - Seavitt, J R
AU  - Seavitt JR
FAU - Noll, M E
AU  - Noll ME
FAU - White, L S
AU  - White LS
FAU - Pingel, J T
AU  - Pingel JT
FAU - Thomas, M L
AU  - Thomas ML
LA  - eng
GR  - AI26363/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Germany
TA  - Eur J Immunol
JT  - European journal of immunology
JID - 1273201
RN  - 0 (Receptors, Antigen)
RN  - EC 2.7.10.2 (src-Family Kinases)
RN  - EC 3.1.3.48 (Leukocyte Common Antigens)
SB  - IM
MH  - Animals
MH  - B-Lymphocytes/*immunology
MH  - Gene Expression Regulation/immunology
MH  - Leukocyte Common Antigens/genetics/*immunology
MH  - Lymphocyte Activation/genetics/immunology
MH  - Mice
MH  - Mice, Knockout
MH  - Receptors, Antigen/genetics/*immunology
MH  - Signal Transduction/genetics/*immunology
MH  - T-Lymphocytes/*immunology
MH  - src-Family Kinases/immunology
EDAT- 1999/12/22 00:00
MHDA- 1999/12/22 00:01
CRDT- 1999/12/22 00:00
PHST- 1999/12/22 00:00 [pubmed]
PHST- 1999/12/22 00:01 [medline]
PHST- 1999/12/22 00:00 [entrez]
AID - 10.1002/(SICI)1521-4141(199912)29:12<3951::AID-IMMU3951>3.0.CO;2-9 [doi]
PST - ppublish
SO  - Eur J Immunol. 1999 Dec;29(12):3951-5. doi:
      10.1002/(SICI)1521-4141(199912)29:12<3951::AID-IMMU3951>3.0.CO;2-9.