PMID- 10601859
OWN - NLM
STAT- MEDLINE
DCOM- 20000215
LR  - 20190620
IS  - 0014-2956 (Print)
IS  - 0014-2956 (Linking)
VI  - 267
IP  - 1
DP  - 2000 Jan
TI  - Assignment of a single disulfide bridge in rat liver methionine
      adenosyltransferase.
PG  - 132-7
AB  - Rat liver methionine adenosyltransferase incorporated 8 mol of N-ethylmaleimide
      per mol of subunit upon denaturation in the presence of 8 M urea, whereas 10 such
      groups were labelled when dithiothreitol was also included. This observation
      prompted a re-examination of the state of the thiol groups, which was carried out
      using peptide mapping, amino acid analysis and N-terminal sequencing. The results
      obtained revealed a disulfide bridge between Cys35 and Cys61. This disulfide did 
      not appear to be conserved because cysteines homologous to residue 61 do not
      exist in methionine adenosyltransferases of other origins, therefore suggesting
      its importance for the differential aspects of the liver-specific enzyme.
FAU - Martinez-Chantar, M L
AU  - Martinez-Chantar ML
AD  - Instituto de Investigaciones Biomedicas 'Alberto Sols' (CSIC-UAM), Madrid, Spain.
FAU - Pajares, M A
AU  - Pajares MA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Biochem
JT  - European journal of biochemistry
JID - 0107600
RN  - 0 (Disulfides)
RN  - 0 (Nitric Oxide Donors)
RN  - 0 (Peptide Fragments)
RN  - 8W8T17847W (Urea)
RN  - EC 2.5.1.6 (Methionine Adenosyltransferase)
RN  - EC 3.2.1.4 (Cellulase)
RN  - EC 3.4.21.4 (Trypsin)
RN  - K848JZ4886 (Cysteine)
RN  - O3C74ACM9V (Ethylmaleimide)
RN  - T8ID5YZU6Y (Dithiothreitol)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cellulase/metabolism
MH  - Cysteine/metabolism
MH  - Dimerization
MH  - Disulfides/*metabolism
MH  - Dithiothreitol/metabolism
MH  - Ethylmaleimide/metabolism
MH  - Liver/*enzymology
MH  - Methionine Adenosyltransferase/*chemistry/*metabolism
MH  - Molecular Sequence Data
MH  - Nitric Oxide Donors/metabolism
MH  - Peptide Fragments/chemistry/metabolism
MH  - Peptide Mapping
MH  - Protein Denaturation/drug effects
MH  - Protein Structure, Quaternary/drug effects
MH  - Rats
MH  - Sequence Analysis, Protein
MH  - Trypsin/metabolism
MH  - Urea/pharmacology
EDAT- 1999/12/22 09:00
MHDA- 2000/02/19 09:00
CRDT- 1999/12/22 09:00
PHST- 1999/12/22 09:00 [pubmed]
PHST- 2000/02/19 09:00 [medline]
PHST- 1999/12/22 09:00 [entrez]
AID - ejb974 [pii]
AID - 10.1046/j.1432-1327.2000.00974.x [doi]
PST - ppublish
SO  - Eur J Biochem. 2000 Jan;267(1):132-7. doi: 10.1046/j.1432-1327.2000.00974.x.