PMID- 10601847
OWN - NLM
STAT- MEDLINE
DCOM- 20000215
LR  - 20190620
IS  - 0014-2956 (Print)
IS  - 0014-2956 (Linking)
VI  - 267
IP  - 1
DP  - 2000 Jan
TI  - Growth factor-induced promoter activation of murine phospholipase C delta4 gene.
PG  - 28-36
AB  - Phospholipase C delta4 (PLCdelta4) is one of the delta-type PLC isozymes, the
      expression of which is induced in nuclei by treatment with serum and also in some
      cancer cells. We isolated and analyzed a promoter region of the murine PLCdelta4 
      gene. DNA sequence analysis showed that this region is GC-rich and has no TATA
      box, and the region from -143 to -127 was found, by luciferase activity and gel
      mobility-shift assay, to be essential for transcription of PLCdelta4. We also
      found that the promoter activity of PLCdelta4 was stimulated by treatment with
      growth factors such as bradykinin, lysophosphatidic acid, and Ca2+ ionophore in
      addition to serum. In parallel, we detected PLCdelta4 mRNA induction and an
      increase in complex formation of the promoter region and nuclear protein from
      HeLa cells on stimulation with these growth factors. Finally, we found that
      trapping the growth factor-induced cytoplasmic Ca2+-inhibited activation of the
      promoter activity and protein induction in nuclei. These results show that
      PLCdelta4 may have an important role in nuclei in response to growth factors, and
      its expression may be partially regulated by an increase in cytoplasmic Ca2+.
FAU - Fukami, K
AU  - Fukami K
AD  - Department of Biochemistry, Institute of Medical Science, University of Tokyo,
      Japan. kfukami@ims.u-tokyo.ac.jp
FAU - Takenaka, K
AU  - Takenaka K
FAU - Nagano, K
AU  - Nagano K
FAU - Takenawa, T
AU  - Takenawa T
LA  - eng
SI  - GENBANK/AF125974
SI  - GENBANK/AF125975
SI  - GENBANK/AF125976
SI  - GENBANK/AF125977
SI  - GENBANK/AF125978
SI  - GENBANK/AF125979
SI  - GENBANK/AF125980
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Biochem
JT  - European journal of biochemistry
JID - 0107600
RN  - 0 (Blood Proteins)
RN  - 0 (Chelating Agents)
RN  - 0 (Growth Substances)
RN  - 0 (Ionophores)
RN  - 0 (Isoenzymes)
RN  - 0 (Lysophospholipids)
RN  - 0 (Platelet-Derived Growth Factor)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
RN  - 9007-49-2 (DNA)
RN  - EC 3.1.4.- (Type C Phospholipases)
RN  - EC 3.1.4.11 (PLCD4 protein, human)
RN  - EC 3.1.4.11 (Phospholipase C delta)
RN  - EC 3.1.4.11 (Plcd4 protein, mouse)
RN  - S8TIM42R2W (Bradykinin)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Blood Proteins/pharmacology
MH  - Bradykinin/pharmacology
MH  - Calcium/antagonists & inhibitors/metabolism
MH  - Calcium Signaling/drug effects
MH  - Cell Line
MH  - Chelating Agents/pharmacology
MH  - DNA/genetics/metabolism
MH  - Gene Expression Regulation, Enzymologic/*drug effects
MH  - Growth Substances/*pharmacology
MH  - HeLa Cells
MH  - Humans
MH  - Insulin-Like Growth Factor I/pharmacology
MH  - Ionophores/pharmacology
MH  - Isoenzymes/*genetics
MH  - Lysophospholipids/pharmacology
MH  - Mice
MH  - Molecular Sequence Data
MH  - Phospholipase C delta
MH  - Platelet-Derived Growth Factor/pharmacology
MH  - Promoter Regions, Genetic/*genetics
MH  - Response Elements/genetics
MH  - Sequence Deletion/genetics
MH  - Transcriptional Activation/*drug effects
MH  - Type C Phospholipases/*genetics
EDAT- 1999/12/22 09:00
MHDA- 2000/02/19 09:00
CRDT- 1999/12/22 09:00
PHST- 1999/12/22 09:00 [pubmed]
PHST- 2000/02/19 09:00 [medline]
PHST- 1999/12/22 09:00 [entrez]
AID - ejb943 [pii]
AID - 10.1046/j.1432-1327.2000.00943.x [doi]
PST - ppublish
SO  - Eur J Biochem. 2000 Jan;267(1):28-36. doi: 10.1046/j.1432-1327.2000.00943.x.