PMID- 10601738
OWN - NLM
STAT- MEDLINE
DCOM- 20000210
LR  - 20101118
IS  - 0945-053X (Print)
IS  - 0945-053X (Linking)
VI  - 18
IP  - 5
DP  - 1999 Oct
TI  - Expression and characterization of the IPM 150 gene (IMPG1) product, a novel
      human photoreceptor cell-associated chondroitin-sulfate proteoglycan.
PG  - 509-18
AB  - The interphotoreceptor matrix (IPM) occupies the extracellular space between the 
      apical surface of the retinal pigmented epithelium and the external limiting
      membrane of the neural retina. This space contains two chondroitin sulfate
      proteoglycans, designated IPM 150 and IPM 200, which are likely to effect retinal
      adhesion and photoreceptor survival. In an effort to characterize human IPM 150, 
      several cDNA clones encoding its core protein have been isolated from a human
      retinal cDNA library. Translation of overlapping cDNA sequences yields a novel
      core protein with a predicted molecular mass of 89.3 kDa. Northern and dot-blot
      analyses as well as the isolation of expressed sequence tags demonstrate that IPM
      150 mRNA is expressed not only in the neural retina but also in several other
      non-ocular tissues. In situ hybridization analyses indicate that, in the eye, IPM
      150 mRNA is expressed specifically by cone and rod photoreceptor cells.
      Characterization of IPM 150 proteoglycan core protein and identification of its
      site of synthesis are important steps towards understanding the architecture and 
      biology of the IPM.
FAU - Kuehn, M H
AU  - Kuehn MH
AD  - Department of Ophthalmology and Visual Sciences, The University of Iowa, Iowa
      52240, USA.
FAU - Hageman, G S
AU  - Hageman GS
LA  - eng
SI  - GENBANK/AF047492
GR  - EY06463/EY/NEI NIH HHS/United States
GR  - EY15151/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Matrix Biol
JT  - Matrix biology : journal of the International Society for Matrix Biology
JID - 9432592
RN  - 0 (Chondroitin Sulfate Proteoglycans)
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Eye Proteins)
RN  - 0 (Glycoproteins)
RN  - 0 (IMPG1 protein, human)
RN  - 0 (Proteoglycans)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Chondroitin Sulfate Proteoglycans/*genetics/*metabolism
MH  - Cloning, Molecular
MH  - DNA Primers/genetics
MH  - DNA, Complementary/genetics/isolation & purification
MH  - Extracellular Matrix/*metabolism
MH  - *Extracellular Matrix Proteins
MH  - *Eye Proteins
MH  - Gene Expression
MH  - Glycoproteins/*genetics/*metabolism
MH  - Humans
MH  - In Situ Hybridization
MH  - Macaca fascicularis
MH  - Molecular Sequence Data
MH  - Photoreceptor Cells, Vertebrate/*metabolism
MH  - *Proteoglycans
MH  - RNA, Messenger/genetics/metabolism
EDAT- 1999/12/22 00:00
MHDA- 1999/12/22 00:01
CRDT- 1999/12/22 00:00
PHST- 1999/12/22 00:00 [pubmed]
PHST- 1999/12/22 00:01 [medline]
PHST- 1999/12/22 00:00 [entrez]
AID - S0945-053X(99)00043-8 [pii]
PST - ppublish
SO  - Matrix Biol. 1999 Oct;18(5):509-18.