PMID- 10601736
OWN - NLM
STAT- MEDLINE
DCOM- 20000210
LR  - 20191210
IS  - 0945-053X (Print)
IS  - 0945-053X (Linking)
VI  - 18
IP  - 5
DP  - 1999 Oct
TI  - Distribution of cartilage molecules in the developing mouse joint.
PG  - 487-97
AB  - This study describes the precise spatial and temporal patterns of protein
      distribution for aggrecan, fibromodulin, cartilage oligomeric matrix protein
      (COMP) and cartilage matrix protein (CMP) in the developing mouse limb with
      particular attention to those cells destined to form articular chondrocytes in
      comparison to those cells destined to form a mineralized tissue and become
      replaced by bone. Mouse glenohumeral joints from fetal mice (12-18 days post
      coitus (dpc) to the young adult (37 days after birth) were immunostained with
      antibodies specific for these molecules. Aggrecan staining defined the general
      chondrocytic phenotype, whether articular or transient. Fibromodulin was
      associated with prechondrocytic mesenchymal cells in the interzone prior to joint
      cavitation and with the mesenchymal cells of the perichondrium or the periosteum 
      encapsulating the joint elements of the maturing and young adult limb. Staining
      was most intense around developing articular chondrocytes and much less abundant 
      or absent in those differentiating cells along the anlage. CMP showed an almost
      reciprocal staining pattern to fibromodulin and was not detected in the matrix
      surrounding articular chondrocytes. COMP was not detected in the cells at the
      articular surface prior to cavitation but by 18 dpc, as coordinated movement of
      the mouse forelimb intensifies, staining for COMP was most intense around the
      maturing articular chondrocytes. These results show that the cells that
      differentiate into articular chondrocytes elaborate an extracellular matrix
      distinct from those cells that are destined to form bone. Fibromodulin may
      function in the early genesis of articular cartilage and COMP may be associated
      with elaboration of a weight-bearing chondrocyte matrix.
FAU - Murphy, J M
AU  - Murphy JM
AD  - Osiris Therapeutics Inc., Baltimore, MD 21231, USA.
FAU - Heinegard, R
AU  - Heinegard R
FAU - McIntosh, A
AU  - McIntosh A
FAU - Sterchi, D
AU  - Sterchi D
FAU - Barry, F P
AU  - Barry FP
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Matrix Biol
JT  - Matrix biology : journal of the International Society for Matrix Biology
JID - 9432592
RN  - 0 (Acan protein, mouse)
RN  - 0 (Aggrecans)
RN  - 0 (Carrier Proteins)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Fmod protein, mouse)
RN  - 0 (Glycoproteins)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Matn1 protein, mouse)
RN  - 0 (Matrilin Proteins)
RN  - 0 (Proteoglycans)
RN  - 126468-95-9 (Fibromodulin)
SB  - IM
MH  - Aggrecans
MH  - Animals
MH  - Animals, Newborn
MH  - Carrier Proteins/metabolism
MH  - Cartilage, Articular/embryology/*growth & development/*metabolism
MH  - Chondrocytes/metabolism
MH  - Extracellular Matrix Proteins/*metabolism
MH  - Female
MH  - Fibromodulin
MH  - Glycoproteins/metabolism
MH  - Humerus/embryology/growth & development/metabolism
MH  - Immunohistochemistry
MH  - Joints/embryology/growth & development/metabolism
MH  - Lectins, C-Type
MH  - Matrilin Proteins
MH  - Mice
MH  - Pregnancy
MH  - Proteoglycans/metabolism
MH  - Scapula/embryology/growth & development/metabolism
EDAT- 1999/12/22 00:00
MHDA- 1999/12/22 00:01
CRDT- 1999/12/22 00:00
PHST- 1999/12/22 00:00 [pubmed]
PHST- 1999/12/22 00:01 [medline]
PHST- 1999/12/22 00:00 [entrez]
AID - S0945053X99000426 [pii]
AID - 10.1016/s0945-053x(99)00042-6 [doi]
PST - ppublish
SO  - Matrix Biol. 1999 Oct;18(5):487-97. doi: 10.1016/s0945-053x(99)00042-6.