PMID- 10601734
OWN - NLM
STAT- MEDLINE
DCOM- 20000210
LR  - 20191103
IS  - 0945-053X (Print)
IS  - 0945-053X (Linking)
VI  - 18
IP  - 5
DP  - 1999 Oct
TI  - Sequence, recombinant expression and tissue localization of two novel
      extracellular matrix proteins, fibulin-3 and fibulin-4.
PG  - 469-80
AB  - Fibulin-1 and fibulin-2 have previously been identified as basement membrane and 
      microfibrillar proteins with a broad binding repertoire for other extracellular
      ligands. Here we report on the cloning and sequence analysis of human fibulin-3
      (487 residues), also known as protein S1-5, and fibulin-4 (443 residues). These
      novel members of this protein family are most closely related to fibulin-1C. They
      consist of a C-terminal globular domain III, also shared by the fibrillins, a
      central rod-like element composed of five calcium-binding epidermal growth
      factor-like (EG) modules (domain II) and an N-terminal interrupted EG module
      (domain I) which replaces the anaphylatoxin-like modules of the other fibulins.
      This predicted domain structure was supported by electron microscopy of
      fibulin-4, which demonstrated short rods. Northern blots showed that both novel
      fibulins are expressed in several human tissues to a variable extent and that
      they are up-regulated in quiescent fibroblasts. Specific antibodies which were
      raised against each of the novel fibulins did not cross-react with fibulin-1.
      Immunohistology of adult mouse tissues showed that fibulin-3, fibulin-4 and
      fibulin-1 have overlapping but distinct extracellular tissue localizations. A
      particularly prominent feature was the staining of variable sets of large and
      small blood vessels.
FAU - Giltay, R
AU  - Giltay R
AD  - Max-Planck-Institut fur Biochemie, Martinsried, Germany.
FAU - Timpl, R
AU  - Timpl R
FAU - Kostka, G
AU  - Kostka G
LA  - eng
SI  - GENBANK/AJ132819
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Matrix Biol
JT  - Matrix biology : journal of the International Society for Matrix Biology
JID - 9432592
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - 0 (fibulin)
RN  - 0 (fibulin 2)
RN  - 0 (fibulin-4)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Calcium-Binding Proteins/chemistry/genetics/metabolism
MH  - Cloning, Molecular
MH  - DNA Primers/genetics
MH  - DNA, Complementary/genetics
MH  - Extracellular Matrix Proteins/chemistry/*genetics/*metabolism
MH  - Fluorescent Antibody Technique
MH  - Gene Expression
MH  - Humans
MH  - Mice
MH  - Microscopy, Electron
MH  - Molecular Sequence Data
MH  - RNA, Messenger/genetics/metabolism
MH  - Recombinant Proteins/chemistry/genetics/metabolism
MH  - Sequence Homology, Amino Acid
MH  - Tissue Distribution
EDAT- 1999/12/22 00:00
MHDA- 1999/12/22 00:01
CRDT- 1999/12/22 00:00
PHST- 1999/12/22 00:00 [pubmed]
PHST- 1999/12/22 00:01 [medline]
PHST- 1999/12/22 00:00 [entrez]
AID - S0945-053X(99)00038-4 [pii]
AID - 10.1016/s0945-053x(99)00038-4 [doi]
PST - ppublish
SO  - Matrix Biol. 1999 Oct;18(5):469-80. doi: 10.1016/s0945-053x(99)00038-4.