PMID- 10601340
OWN - NLM
STAT- MEDLINE
DCOM- 20000111
LR  - 20191023
IS  - 0021-9525 (Print)
IS  - 0021-9525 (Linking)
VI  - 147
IP  - 6
DP  - 1999 Dec 13
TI  - Microtubule actin cross-linking factor (MACF): a hybrid of dystonin and
      dystrophin that can interact with the actin and microtubule cytoskeletons.
PG  - 1275-86
AB  - We cloned and characterized a full-length cDNA of mouse actin cross-linking
      family 7 (mACF7) by sequential rapid amplification of cDNA ends-PCR. The
      completed mACF7 cDNA is 17 kb and codes for a 608-kD protein. The closest
      relative of mACF7 is the Drosophila protein Kakapo, which shares similar
      architecture with mACF7. mACF7 contains a putative actin-binding domain and a
      plakin-like domain that are highly homologous to dystonin (BPAG1-n) at its NH(2) 
      terminus. However, unlike dystonin, mACF7 does not contain a coiled-coil rod
      domain; instead, the rod domain of mACF7 is made up of 23 dystrophin-like
      spectrin repeats. At its COOH terminus, mACF7 contains two putative EF-hand
      calcium-binding motifs and a segment homologous to the growth arrest-specific
      protein, Gas2. In this paper, we demonstrate that the NH(2)-terminal
      actin-binding domain of mACF7 is functional both in vivo and in vitro. More
      importantly, we found that the COOH-terminal domain of mACF7 interacts with and
      stabilizes microtubules. In transfected cells full-length mACF7 can associate not
      only with actin but also with microtubules. Hence, we suggest a modified name:
      MACF (microtubule actin cross-linking factor). The properties of MACF are
      consistent with the observation that mutations in kakapo cause disorganization of
      microtubules in epidermal muscle attachment cells and some sensory neurons.
FAU - Leung, C L
AU  - Leung CL
AD  - Department of Pathology and Department of Anatomy and Cell Biology, Columbia
      University College of Physicians and Surgeons, New York, New York 10032, USA.
FAU - Sun, D
AU  - Sun D
FAU - Zheng, M
AU  - Zheng M
FAU - Knowles, D R
AU  - Knowles DR
FAU - Liem, R K
AU  - Liem RK
LA  - eng
SI  - GENBANK/AF150755
GR  - AG00189/AG/NIA NIH HHS/United States
GR  - NS15182/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Actins)
RN  - 0 (Carrier Proteins)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (DST protein, human)
RN  - 0 (Drosophila Proteins)
RN  - 0 (Dst protein, mouse)
RN  - 0 (Dystonin)
RN  - 0 (Dystrophin)
RN  - 0 (MACF1 protein, human)
RN  - 0 (Macf1 protein, mouse)
RN  - 0 (Microfilament Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (shot protein, Drosophila)
SB  - IM
MH  - Actin Cytoskeleton/metabolism
MH  - Actins/*metabolism
MH  - Amino Acid Motifs
MH  - Amino Acid Sequence
MH  - Animals
MH  - COS Cells
MH  - *Carrier Proteins
MH  - Cloning, Molecular
MH  - Cytoskeletal Proteins/*chemistry
MH  - Cytoskeleton/*metabolism
MH  - *Drosophila Proteins
MH  - Dystonin
MH  - Dystrophin/*chemistry
MH  - Embryo, Mammalian/metabolism
MH  - Humans
MH  - Mice
MH  - Microfilament Proteins/chemistry/genetics/*metabolism
MH  - Microtubules/*metabolism
MH  - Molecular Sequence Data
MH  - Nerve Tissue Proteins/*chemistry
MH  - Protein Binding
MH  - Protein Structure, Secondary
MH  - RNA, Messenger/analysis/genetics
MH  - Recombinant Fusion Proteins/metabolism
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
PMC - PMC2168091
EDAT- 1999/12/22 00:00
MHDA- 1999/12/22 00:01
CRDT- 1999/12/22 00:00
PHST- 1999/12/22 00:00 [pubmed]
PHST- 1999/12/22 00:01 [medline]
PHST- 1999/12/22 00:00 [entrez]
AID - 10.1083/jcb.147.6.1275 [doi]
PST - ppublish
SO  - J Cell Biol. 1999 Dec 13;147(6):1275-86. doi: 10.1083/jcb.147.6.1275.