PMID- 10601336
OWN - NLM
STAT- MEDLINE
DCOM- 20000111
LR  - 20191210
IS  - 0021-9525 (Print)
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Linking)
VI  - 147
IP  - 6
DP  - 1999 Dec 13
TI  - Role for Drs2p, a P-type ATPase and potential aminophospholipid translocase, in 
      yeast late Golgi function.
PG  - 1223-36
AB  - ADP-ribosylation factor appears to regulate the budding of both COPI and 
      clathrin-coated transport vesicles from Golgi membranes. An arf1Delta synthetic 
      lethal screen identified SWA3/DRS2, which encodes an integral membrane P-type 
      ATPase and potential aminophospholipid translocase (or flippase). The drs2 null 
      allele is also synthetically lethal with clathrin heavy chain (chc1) 
      temperature-sensitive alleles, but not with mutations in COPI subunits or other 
      SEC genes tested. Consistent with these genetic analyses, we found that the 
      drs2Delta mutant exhibits late Golgi defects that may result from a loss of 
      clathrin function at this compartment. These include a defect in the 
      Kex2-dependent processing of pro-alpha-factor and the accumulation of abnormal 
      Golgi cisternae. Moreover, we observed a marked reduction in clathrin-coated 
      vesicles that can be isolated from the drs2Delta cells. Subcellular fractionation 
      and immunofluorescence analysis indicate that Drs2p localizes to late Golgi 
      membranes containing Kex2p. These observations indicate a novel role for a P-type 
      ATPase in late Golgi function and suggest a possible link between membrane 
      asymmetry and clathrin function at the Golgi complex.
FAU - Chen, C Y
AU  - Chen CY
AD  - Department of Molecular Biology, Vanderbilt University, Nashville, Tennessee 
      37235, USA.
FAU - Ingram, M F
AU  - Ingram MF
FAU - Rosal, P H
AU  - Rosal PH
FAU - Graham, T R
AU  - Graham TR
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Carrier Proteins)
RN  - 0 (Clathrin)
RN  - 0 (DRS2 protein, S cerevisiae)
RN  - 0 (Fungal Proteins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Peptides)
RN  - 0 (Phospholipid Transfer Proteins)
RN  - 0 (Protein Precursors)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 114899-12-6 (Clathrin Heavy Chains)
RN  - 30KYC7MIAI (Aspartic Acid)
RN  - 61194-02-3 (Mating Factor)
RN  - EC 2.4.1.- (MNN1 protein, S cerevisiae)
RN  - EC 2.4.1.- (Mannosyltransferases)
RN  - EC 3.4.21.- (Proprotein Convertases)
RN  - EC 3.4.21.- (Subtilisins)
RN  - EC 3.4.21.61 (KEX2 protein, S cerevisiae)
RN  - EC 3.6.5.2 (ADP-Ribosylation Factor 1)
RN  - EC 7.2.2.10 (Calcium-Transporting ATPases)
SB  - IM
MH  - ADP-Ribosylation Factor 1/genetics/physiology
MH  - Amino Acid Sequence
MH  - Aspartic Acid/genetics/metabolism
MH  - Biological Transport
MH  - Calcium-Transporting ATPases/chemistry/genetics/*metabolism
MH  - Carrier Proteins/chemistry/genetics/*metabolism
MH  - Clathrin/genetics/physiology
MH  - Clathrin Heavy Chains
MH  - Coated Vesicles/metabolism
MH  - Endosomes/metabolism
MH  - Fungal Proteins/chemistry/genetics/*metabolism
MH  - Genes, Fungal/genetics/physiology
MH  - Genes, Lethal/genetics
MH  - Golgi Apparatus/enzymology/*metabolism
MH  - Intracellular Membranes/enzymology/metabolism
MH  - *Mannosyltransferases
MH  - Mating Factor
MH  - Membrane Glycoproteins/metabolism
MH  - Membrane Proteins/chemistry/genetics/*metabolism
MH  - Molecular Sequence Data
MH  - Mutation/genetics
MH  - Organelles/metabolism
MH  - Peptides/metabolism
MH  - Phenotype
MH  - *Phospholipid Transfer Proteins
MH  - *Proprotein Convertases
MH  - Protein Precursors/metabolism
MH  - Saccharomyces cerevisiae/*cytology/*enzymology/genetics/metabolism
MH  - *Saccharomyces cerevisiae Proteins
MH  - Subtilisins/metabolism
PMC - PMC2168089
EDAT- 1999/12/22 00:00
MHDA- 1999/12/22 00:01
CRDT- 1999/12/22 00:00
PHST- 1999/12/22 00:00 [pubmed]
PHST- 1999/12/22 00:01 [medline]
PHST- 1999/12/22 00:00 [entrez]
AID - 9905038 [pii]
AID - 10.1083/jcb.147.6.1223 [doi]
PST - ppublish
SO  - J Cell Biol. 1999 Dec 13;147(6):1223-36. doi: 10.1083/jcb.147.6.1223.