PMID- 10601332
OWN - NLM
STAT- MEDLINE
DCOM- 20000111
LR  - 20190508
IS  - 0021-9525 (Print)
IS  - 0021-9525 (Linking)
VI  - 147
IP  - 6
DP  - 1999 Dec 13
TI  - The A-kinase-anchoring protein AKAP95 is a multivalent protein with a key role in
      chromatin condensation at mitosis.
PG  - 1167-80
AB  - Protein kinase A (PKA) and the nuclear A-kinase-anchoring protein AKAP95 have
      previously been shown to localize in separate compartments in interphase but
      associate at mitosis. We demonstrate here a role for the mitotic AKAP95-PKA
      complex. In HeLa cells, AKAP95 is associated with the nuclear matrix in
      interphase and redistributes mostly into a chromatin fraction at mitosis. In a
      cytosolic extract derived from mitotic cells, AKAP95 recruits the RIIalpha
      regulatory subunit of PKA onto chromatin. Intranuclear immunoblocking of AKAP95
      inhibits chromosome condensation at mitosis and in mitotic extract in a
      PKA-independent manner. Immunodepletion of AKAP95 from the extract or
      immunoblocking of AKAP95 at metaphase induces premature chromatin decondensation.
      Condensation is restored in vitro by a recombinant AKAP95 fragment comprising the
      306-carboxy-terminal amino acids of the protein. Maintenance of condensed
      chromatin requires PKA binding to chromatin-associated AKAP95 and cAMP signaling 
      through PKA. Chromatin-associated AKAP95 interacts with Eg7, the human homologue 
      of Xenopus pEg7, a component of the 13S condensin complex. Moreover,
      immunoblocking nuclear AKAP95 inhibits the recruitment of Eg7 to chromatin in
      vitro. We propose that AKAP95 is a multivalent molecule that in addition to
      anchoring a cAMP/PKA-signaling complex onto chromosomes, plays a role in
      regulating chromosome structure at mitosis.
FAU - Collas, P
AU  - Collas P
AD  - Institute of Medical Biochemistry, Faculty of Medicine, University of Oslo,
      Blindern, 0317 Oslo, Norway. philippe.collas@basalmed.uio.no
FAU - Le Guellec, K
AU  - Le Guellec K
FAU - Tasken, K
AU  - Tasken K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (AKAP8L protein, human)
RN  - 0 (Antibodies)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Cell Extracts)
RN  - 0 (Chromatin)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Eg7 protein, Xenopus)
RN  - 0 (Egg Proteins)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Peptide Fragments)
RN  - 0 (Xenopus Proteins)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
RN  - SH1WY3R615 (Nocodazole)
SB  - IM
MH  - Antibodies/pharmacology
MH  - Cell Cycle/drug effects
MH  - Cell Cycle Proteins/metabolism
MH  - Cell Extracts
MH  - Chromatin/chemistry/genetics/*metabolism
MH  - Chromosomes, Human/chemistry/drug effects/genetics/*metabolism
MH  - Cyclic AMP/antagonists & inhibitors/pharmacology
MH  - Cyclic AMP-Dependent Protein Kinases/antagonists &
      inhibitors/chemistry/*metabolism
MH  - DNA-Binding Proteins/antagonists & inhibitors/chemistry/genetics/*metabolism
MH  - Egg Proteins/metabolism
MH  - Fluorescent Antibody Technique
MH  - HeLa Cells
MH  - Humans
MH  - Intracellular Signaling Peptides and Proteins
MH  - *Mitosis/drug effects
MH  - Nocodazole/pharmacology
MH  - Nuclear Matrix/drug effects/metabolism
MH  - Nuclear Proteins/antagonists & inhibitors/chemistry/genetics/*metabolism
MH  - Peptide Fragments/chemistry/genetics/metabolism
MH  - Precipitin Tests
MH  - Protein Binding
MH  - Signal Transduction/drug effects
MH  - *Xenopus Proteins
PMC - PMC2168084
EDAT- 1999/12/22 00:00
MHDA- 1999/12/22 00:01
CRDT- 1999/12/22 00:00
PHST- 1999/12/22 00:00 [pubmed]
PHST- 1999/12/22 00:01 [medline]
PHST- 1999/12/22 00:00 [entrez]
AID - 10.1083/jcb.147.6.1167 [doi]
PST - ppublish
SO  - J Cell Biol. 1999 Dec 13;147(6):1167-80. doi: 10.1083/jcb.147.6.1167.