PMID- 10601261
OWN - NLM
STAT- MEDLINE
DCOM- 20000113
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 52
DP  - 1999 Dec 24
TI  - Isolation and cDNA cloning of a novel galanin-like peptide (GALP) from porcine
      hypothalamus.
PG  - 37041-5
AB  - Galanin is a widely distributed neuropeptide with a variety of physiological
      functions. Three galanin receptor subtypes, GALR1, GALR2, and GALR3, have been
      reported. We isolated a novel galanin-like peptide (GALP) from porcine
      hypothalamus by observing its activity for increasing [(35)S]GTPgammaS binding to
      a membrane preparation of GALR2-transfected cells. The peptide had 60 amino acid 
      residues and a non-amidated C terminus. The amino acid sequence of GALP-(9-21)
      was completely identical to that of galanin-(1-13). A cloned porcine GALP cDNA
      indicated that GALP was processed from a 120-amino acid GALP precursor protein.
      The structures of rat and human GALP-(1-60) were deduced from cloned cDNA, which 
      indicated that the amino acid sequences 1-24 and 41-53 were highly conserved
      between humans, rats, and pigs. Receptor binding studies revealed that porcine
      GALP-(1-60) had a high affinity for the GALR2 receptor (IC(50) = 0.24 nM) and a
      lower affinity for the GALR1 receptor (IC(50) = 4.3 nM). In contrast, galanin
      showed high affinity for the GALR1 (IC(50) = 0.097 nM) and GALR2 receptors
      (IC(50) = 0.48 nM). GALP is therefore an endogenous ligand that preferentially
      binds the GALR2 receptor, whereas galanin is relatively non-selective.
FAU - Ohtaki, T
AU  - Ohtaki T
AD  - Discovery Research Laboratories I, Pharmaceutical Discovery Research Division,
      Takeda Chemical Industries, Ltd., Wadai 10, Tsukuba, Ibaraki 300-4293, Japan.
      Ohtaki_Tetsuya@takeda.co.jp
FAU - Kumano, S
AU  - Kumano S
FAU - Ishibashi, Y
AU  - Ishibashi Y
FAU - Ogi, K
AU  - Ogi K
FAU - Matsui, H
AU  - Matsui H
FAU - Harada, M
AU  - Harada M
FAU - Kitada, C
AU  - Kitada C
FAU - Kurokawa, T
AU  - Kurokawa T
FAU - Onda, H
AU  - Onda H
FAU - Fujino, M
AU  - Fujino M
LA  - eng
SI  - GENBANK/AF188490
SI  - GENBANK/AF188491
SI  - GENBANK/AF188492
SI  - GENBANK/AF188493
PT  - Journal Article
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA, Complementary)
RN  - 0 (Receptors, Galanin)
RN  - 0 (Receptors, Neuropeptide)
RN  - 88813-36-9 (Galanin)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - CHO Cells
MH  - Cloning, Molecular
MH  - Cricetinae
MH  - DNA, Complementary/chemistry/*isolation & purification
MH  - Galanin/genetics/*isolation & purification
MH  - Humans
MH  - Hypothalamus/*chemistry
MH  - Molecular Sequence Data
MH  - Rats
MH  - Receptors, Galanin
MH  - Receptors, Neuropeptide/agonists
MH  - Swine
EDAT- 1999/12/22 00:00
MHDA- 1999/12/22 00:01
CRDT- 1999/12/22 00:00
PHST- 1999/12/22 00:00 [pubmed]
PHST- 1999/12/22 00:01 [medline]
PHST- 1999/12/22 00:00 [entrez]
AID - 10.1074/jbc.274.52.37041 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Dec 24;274(52):37041-5. doi: 10.1074/jbc.274.52.37041.