PMID- 10600773
OWN - NLM
STAT- MEDLINE
DCOM- 20000120
LR  - 20180920
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 277
IP  - 6
DP  - 1999 Dec
TI  - Molecular cloning and functional characterization of KCC3, a new K-Cl
      cotransporter.
PG  - C1210-9
LID - 10.1152/ajpcell.1999.277.6.C1210 [doi]
AB  - We isolated and characterized a novel K-Cl cotransporter, KCC3, from human
      placenta. The deduced protein contains 1,150 amino acids. KCC3 shares 75-76%
      identity at the amino acid level with human, pig, rat, and rabbit KCC1 and 67%
      identity with rat KCC2. KCC3 is 40 and 33% identical to two Caenorhabditis
      elegans K-Cl cotransporters and approximately 20% identical to other members of
      the cation-chloride cotransporter family (CCC), two Na-K-Cl cotransporters
      (NKCC1, NKCC2), and the Na-Cl cotransporter (NCC). Hydropathy analysis indicates 
      a typical KCC topology with 12 transmembrane domains, a large extracellular loop 
      between transmembrane domains 5 and 6 (unique to KCCs), and large NH(2) and COOH 
      termini. KCC3 is predominantly expressed in kidney, heart, and brain, and is also
      expressed in skeletal muscle, placenta, lung, liver, and pancreas. KCC3 was
      localized to chromosome 15. KCC3 transiently expressed in human embryonic kidney 
      (HEK)-293 cells fulfilled three criteria for increased expression of K-Cl
      cotransport: stimulation of cotransport by swelling, treatment with
      N-ethylmaleimide, or treatment with staurosporine.
FAU - Race, J E
AU  - Race JE
AD  - Renal Division, Department of Medicine, State University of New York Health
      Science Center, Syracuse 13210, New York.
FAU - Makhlouf, F N
AU  - Makhlouf FN
FAU - Logue, P J
AU  - Logue PJ
FAU - Wilson, F H
AU  - Wilson FH
FAU - Dunham, P B
AU  - Dunham PB
FAU - Holtzman, E J
AU  - Holtzman EJ
LA  - eng
SI  - GENBANK/AF116242
GR  - R37-33640/PHS HHS/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA Primers)
RN  - 0 (KCC3 protein, rat)
RN  - 0 (SLC12A6 protein, human)
RN  - 0 (Sulfhydryl Reagents)
RN  - 0 (Symporters)
RN  - 4R7X1O2820 (Chlorine)
RN  - O3C74ACM9V (Ethylmaleimide)
RN  - RWP5GA015D (Potassium)
SB  - IM
MH  - Biological Transport/drug effects/physiology
MH  - Carrier Proteins/chemistry/*genetics
MH  - Cell Line
MH  - Chlorine/metabolism
MH  - Chromosome Mapping
MH  - Cloning, Molecular
MH  - DNA Primers
MH  - Ethylmaleimide/pharmacology
MH  - Gene Expression/physiology
MH  - Humans
MH  - Kidney/cytology
MH  - Molecular Sequence Data
MH  - Osmosis
MH  - Phylogeny
MH  - Placenta/*chemistry
MH  - Potassium/metabolism
MH  - Protein Structure, Tertiary
MH  - Sequence Homology, Amino Acid
MH  - Sulfhydryl Reagents/pharmacology
MH  - *Symporters
MH  - Transfection
EDAT- 1999/12/22 00:00
MHDA- 1999/12/22 00:01
CRDT- 1999/12/22 00:00
PHST- 1999/12/22 00:00 [pubmed]
PHST- 1999/12/22 00:01 [medline]
PHST- 1999/12/22 00:00 [entrez]
AID - 10.1152/ajpcell.1999.277.6.C1210 [doi]
PST - ppublish
SO  - Am J Physiol. 1999 Dec;277(6):C1210-9. doi: 10.1152/ajpcell.1999.277.6.C1210.