PMID- 10600646
OWN - NLM
STAT- MEDLINE
DCOM- 20000214
LR  - 20181113
IS  - 0264-6021 (Print)
IS  - 0264-6021 (Linking)
VI  - 345 Pt 1
DP  - 2000 Jan 1
TI  - Molecular cloning and functional characterization of the mouse
      organic-anion-transporting polypeptide 1 (Oatp1) and mapping of the gene to
      chromosome X.
PG  - 115-20
AB  - We have cloned a murine member of the organic-anion-transporting polypeptide
      (Oatp) family of membrane-transport proteins from mouse liver. The cloned cDNA
      insert of 2783 bp with an open reading frame of 2011 bp codes for a
      12-transmembrane 670-amino-acid protein with highest amino acid identity with the
      rat Oatp1. When expressed in Xenopus laevis oocytes, the mouse Oatp exhibited the
      same substrate specificity as the rat Oatp1. Besides the common Oatp substrates
      bromosulphophthalein, taurocholate, oestrone 3-sulphate and ouabain, the new
      mouse Oatp also mediates transport of the Oatp1-specific
      magnetic-resonance-imaging agent gadoxetate. The Oatp2-specific cardiac glycoside
      digoxin, however, is not transported. Kinetic analyses performed for taurocholate
      and oestrone 3-sulphate revealed apparent K(m) values of 12 microM and 5 microM
      respectively. Northern-blot analysis demonstrated a predominant expression in the
      liver with an additional moderate expression in the kidney. Taken together, the
      amino acid identity, the functional characteristics and the tissue distribution
      suggest that we have isolated the murine orthologue of the rat Oatp1, and
      consequently the identified protein will be called Oatp1. Using fluorescence in
      situ hybridization, the murine Oatp1 gene was mapped to chromosome XA3-A5.
FAU - Hagenbuch, B
AU  - Hagenbuch B
AD  - Division of Clinical Pharmacology, Department of Medicine, University Hospital,
      CH-8091 Zurich, Switzerland. Bruno.Hagenbuch@access.unizh.ch
FAU - Adler, I D
AU  - Adler ID
FAU - Schmid, T E
AU  - Schmid TE
LA  - eng
SI  - GENBANK/AF148218
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (Anion Transport Proteins)
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA Primers)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Anion Transport Proteins
MH  - Base Sequence
MH  - Carrier Proteins/chemistry/*genetics/*metabolism
MH  - Chromosome Mapping
MH  - Cloning, Molecular
MH  - DNA Primers/genetics
MH  - Female
MH  - Gene Expression
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Mice
MH  - Molecular Sequence Data
MH  - Oocytes/metabolism
MH  - Rats
MH  - Sequence Homology, Amino Acid
MH  - Tissue Distribution
MH  - X Chromosome/genetics
MH  - Xenopus laevis
PMC - PMC1220737
EDAT- 1999/12/22 09:00
MHDA- 2000/02/19 09:00
CRDT- 1999/12/22 09:00
PHST- 1999/12/22 09:00 [pubmed]
PHST- 2000/02/19 09:00 [medline]
PHST- 1999/12/22 09:00 [entrez]
PST - ppublish
SO  - Biochem J. 2000 Jan 1;345 Pt 1:115-20.