PMID- 10600528
OWN - NLM
STAT- MEDLINE
DCOM- 20000118
LR  - 20061115
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 266
IP  - 2
DP  - 1999 Dec 20
TI  - c-Src activates the DNA binding and transcriptional activity of Stat3 molecules: 
      serine 727 is not required for transcriptional activation under certain
      circumstances.
PG  - 481-7
AB  - Stat3 proteins are constitutively activated in cells transformed by v-Src and the
      proteins have been shown to interact directly. Subsequent studies have shown that
      Stat3 is required for cellular transformation of NIH fibroblasts by v-Src,
      suggesting a potential role for Stat3 in aberrant cell growth. Stat3 is
      phosphorylated on a single tyrosine (tyrosine 705) which is required for
      effective dimer formation. An additional phosphorylation event (serine 727) is
      believed to be required for full transcriptional activity of Stat1 and Stat3
      molecules. Here we report that c-Src activates the DNA binding activity of
      Stat3alpha, Stat3beta, and three Stat3 mutants, one in which serine 727 was
      replaced by alanine (Stat3alphaS727A) and C-terminal truncated molecules Delta48 
      and Delta55. Consistent with this finding is a general increase in the tyrosine
      705-phosphorylated Stat3 in cells cotransfected with c-Src. Furthermore,
      transcription from an alpha-2 macroglobulin reporter gene is activated by
      Stat3alphaS727A to the same magnitude as compared to Stat3alpha and Stat3beta in 
      the presence of c-Src. These results suggest that serine 727, contained in a
      consensus MAP kinase recognition site and shown to be the only serine in Stat3
      phosphorylated in epidermal growth factor (EGF) treated cells, is not necessary
      for transcriptional activity comparable to wild-type Stat3alpha or Stat3beta when
      activated by c-Src in COS-7 cells.
CI  - Copyright 1999 Academic Press.
FAU - Schaefer, L K
AU  - Schaefer LK
AD  - Department of Neurosurgery, Department of Molecular Genetics, University of Texas
      M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, Texas 77030, USA.
FAU - Wang, S
AU  - Wang S
FAU - Schaefer, T S
AU  - Schaefer TS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (STAT3 Transcription Factor)
RN  - 0 (Trans-Activators)
RN  - 62229-50-9 (Epidermal Growth Factor)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - COS Cells
MH  - DNA-Binding Proteins/*genetics
MH  - Epidermal Growth Factor/pharmacology
MH  - Genes, Reporter
MH  - Genes, jun/genetics
MH  - Genes, src/*genetics
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Phosphorylation
MH  - STAT3 Transcription Factor
MH  - Sequence Deletion
MH  - Trans-Activators/*genetics
MH  - Transcription, Genetic
MH  - Transfection
EDAT- 1999/12/22 00:00
MHDA- 1999/12/22 00:01
CRDT- 1999/12/22 00:00
PHST- 1999/12/22 00:00 [pubmed]
PHST- 1999/12/22 00:01 [medline]
PHST- 1999/12/22 00:00 [entrez]
AID - 10.1006/bbrc.1999.1853 [doi]
AID - S0006-291X(99)91853-3 [pii]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Dec 20;266(2):481-7. doi:
      10.1006/bbrc.1999.1853.