PMID- 10600472
OWN - NLM
STAT- MEDLINE
DCOM- 20000111
LR  - 20141120
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 265
IP  - 3
DP  - 1999 Nov 30
TI  - SNX5, a new member of the sorting nexin family, binds to the Fanconi anemia
      complementation group A protein.
PG  - 630-5
AB  - The function of the Fanconi anemia complementation group A (FANCA) protein
      remains unclear. To investigate possible protein-protein interactions, we
      performed yeast two-hybrid screening using a FANCA fragment as bait. Sorting
      nexin 5 (SNX5), a new member of the human SNX family, was identified as a
      putative FANCA-binding protein. The interaction between FANCA and SNX5 was
      confirmed by immunoprecipitation studies. All members of the SNX family have a
      characteristic amino acid region termed the phox homology (PX) domain. Deletion
      mutant analysis indicated that the PX domain is not required for binding to
      FANCA. The SNX proteins are thought to play an important role in receptor
      trafficking between organelles. We found that overexpression of SNX5 increased
      FANCA protein levels. Northern blot analysis of SNX5 showed the presence of
      alternatively spliced transcripts and different expression patterns in various
      human cancer cell lines and normal tissues. Further studies are needed to
      elucidate the functional significance of FANCA and SNX5 binding; however, we
      speculate that FANCA may affect SNX5 traffic with cell surface receptors.
CI  - Copyright 1999 Academic Press.
FAU - Otsuki, T
AU  - Otsuki T
AD  - Department of Hematology, Jichi Medical School, Yakushiji 3311-1, Minamikawachi, 
      Kawachi, Tochigi, 329-0498, Japan. tohtsuki@jichi.ac.jp
FAU - Kajigaya, S
AU  - Kajigaya S
FAU - Ozawa, K
AU  - Ozawa K
FAU - Liu, J M
AU  - Liu JM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (FANCA protein, human)
RN  - 0 (Fanconi Anemia Complementation Group A Protein)
RN  - 0 (Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (SNX5 protein, human)
RN  - 0 (Sorting Nexins)
RN  - 0 (Vesicular Transport Proteins)
SB  - IM
MH  - Alternative Splicing
MH  - Amino Acid Sequence
MH  - Binding Sites/genetics
MH  - Carrier Proteins/chemistry/genetics/*metabolism
MH  - *DNA-Binding Proteins
MH  - Fanconi Anemia/genetics/*metabolism
MH  - Fanconi Anemia Complementation Group A Protein
MH  - Humans
MH  - In Vitro Techniques
MH  - Molecular Sequence Data
MH  - Protein Binding
MH  - Proteins/genetics/*metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Sequence Homology, Amino Acid
MH  - Sorting Nexins
MH  - Tissue Distribution
MH  - Tumor Cells, Cultured
MH  - Two-Hybrid System Techniques
MH  - *Vesicular Transport Proteins
EDAT- 1999/12/22 00:00
MHDA- 1999/12/22 00:01
CRDT- 1999/12/22 00:00
PHST- 1999/12/22 00:00 [pubmed]
PHST- 1999/12/22 00:01 [medline]
PHST- 1999/12/22 00:00 [entrez]
AID - 10.1006/bbrc.1999.1731 [doi]
AID - S0006-291X(99)91731-X [pii]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1999 Nov 30;265(3):630-5. doi:
      10.1006/bbrc.1999.1731.