PMID- 10600115
OWN - NLM
STAT- MEDLINE
DCOM- 20000119
LR  - 20190613
IS  - 0006-2960 (Print)
IS  - 0006-2960 (Linking)
VI  - 38
IP  - 50
DP  - 1999 Dec 14
TI  - Purification of porcine brain protein phosphatase 2A leucine carboxyl
      methyltransferase and cloning of the human homologue.
PG  - 16539-47
AB  - The carboxyl methyltransferase, which is claimed to exclusively methylate the
      carboxyl group of the C-terminal leucine residue of the catalytic subunit of
      protein phosphatase 2A (Leu(309)), was purified from porcine brain. On the basis 
      of tryptic peptides, the cDNA encoding the human homologue was cloned. The cDNA
      of this gene encodes for a protein of 334 amino acids with a calculated M(r) of
      38 305 and a predicted pI of 5.72. Database screening reveals the presence of
      this protein in diverse phyla. Sequence analysis shows that the novel
      methyltransferase is distinct from other known protein methyltransferases,
      sharing only sequence motifs supposedly involved in the binding of
      adenosylmethionine. The recombinant protein expressed in bacteria is soluble and 
      the biophysical, catalytic, and immunological properties are indistinguishable
      from the native enzyme. The methylation of PP2A by the recombinant protein is
      restricted to Leu(309) of PP2A(C). No direct effects on phosphatase activity
      changes were observed upon methylation of the dimeric or trimeric forms of PP2A.
FAU - De Baere, I
AU  - De Baere I
AD  - Afdeling Biochemie, Faculteit Geneeskunde, Katholieke Universiteit Leuven, B-3000
      Leuven, Belgium.
FAU - Derua, R
AU  - Derua R
FAU - Janssens, V
AU  - Janssens V
FAU - Van Hoof, C
AU  - Van Hoof C
FAU - Waelkens, E
AU  - Waelkens E
FAU - Merlevede, W
AU  - Merlevede W
FAU - Goris, J
AU  - Goris J
LA  - eng
SI  - GENBANK/AF037601
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochemistry
JT  - Biochemistry
JID - 0370623
RN  - 0 (Recombinant Proteins)
RN  - EC 2.1.1.- (C-terminal leucine protein methyltransferase)
RN  - EC 2.1.1.- (Protein O-Methyltransferase)
RN  - EC 3.1.3.16 (Phosphoprotein Phosphatases)
RN  - EC 3.1.3.16 (Protein Phosphatase 2)
RN  - GMW67QNF9C (Leucine)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Brain/*enzymology
MH  - Cloning, Molecular
MH  - Enzyme Activation
MH  - Escherichia coli/enzymology/genetics
MH  - Humans
MH  - Leucine/metabolism
MH  - Methylation
MH  - Molecular Sequence Data
MH  - Phosphoprotein Phosphatases/*metabolism
MH  - Protein O-Methyltransferase/genetics/immunology/*isolation &
      purification/metabolism
MH  - Protein Phosphatase 2
MH  - Rabbits
MH  - Recombinant Proteins/biosynthesis/isolation & purification/metabolism
MH  - Substrate Specificity
MH  - Swine
EDAT- 1999/12/22 00:00
MHDA- 1999/12/22 00:01
CRDT- 1999/12/22 00:00
PHST- 1999/12/22 00:00 [pubmed]
PHST- 1999/12/22 00:01 [medline]
PHST- 1999/12/22 00:00 [entrez]
AID - bi991646a [pii]
AID - 10.1021/bi991646a [doi]
PST - ppublish
SO  - Biochemistry. 1999 Dec 14;38(50):16539-47. doi: 10.1021/bi991646a.