PMID- 10598593
OWN - NLM
STAT- MEDLINE
DCOM- 20000110
LR  - 20111117
IS  - 0888-8809 (Print)
IS  - 0888-8809 (Linking)
VI  - 13
IP  - 12
DP  - 1999 Dec
TI  - Differential activation of pituitary hormone genes by human Lhx3 isoforms with
      distinct DNA binding properties.
PG  - 2212-25
AB  - Lhx3 is a LIM homeodomain transcription factor essential for pituitary
      development and motor neuron specification in mice. We identified two isoforms of
      human Lhx3, hLhx3a and hLhx3b, which differ in their ability to trans-activate
      pituitary gene targets. These factors are identical within the LIM domains and
      the homeodomain, but differ in their amino-terminal sequences preceding the LIM
      motifs. Both isoforms are localized to the nucleus and are expressed in the adult
      human pituitary, but gene activation studies demonstrate characteristic
      functional differences. Human Lhx3a trans-activated the alpha-glycoprotein
      subunit promoter and a reporter construct containing a high-affinity Lhx3 binding
      site more effectively than the hLhx3b isoform. In addition, hLhx3a synergized
      with the pituitary POU domain factor, Pit-1, to strongly induce transcription of 
      the TSHbeta-subunit gene, while hLhx3b did not. We demonstrate that the
      differences in gene activation properties between hLhx3a and hLhx3b correlate
      with their DNA binding to sites within these genes. The short hLhx3b-specific
      amino-terminal domain inhibits DNA binding and gene activation functions of the
      molecule. These data suggest that isoforms of Lhx3 may play distinct roles during
      development of the mammalian pituitary gland and other neuroendocrine systems.
FAU - Sloop, K W
AU  - Sloop KW
AD  - Department of Biology, Indiana University-Purdue University Indianapolis
      46202-5132, USA.
FAU - Meier, B C
AU  - Meier BC
FAU - Bridwell, J L
AU  - Bridwell JL
FAU - Parker, G E
AU  - Parker GE
FAU - Schiller, A M
AU  - Schiller AM
FAU - Rhodes, S J
AU  - Rhodes SJ
LA  - eng
SI  - GENBANK/AF156888
SI  - GENBANK/AF156889
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Mol Endocrinol
JT  - Molecular endocrinology (Baltimore, Md.)
JID - 8801431
RN  - 0 (DNA, Complementary)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (LIM-Homeodomain Proteins)
RN  - 0 (Lhx3 protein)
RN  - 0 (Pituitary Hormones)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Transcription Factors)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cell Line
MH  - Cloning, Molecular
MH  - DNA/*metabolism
MH  - DNA, Complementary
MH  - Escherichia coli/genetics
MH  - *Gene Expression
MH  - Homeodomain Proteins/genetics/*metabolism/*pharmacology
MH  - Humans
MH  - LIM-Homeodomain Proteins
MH  - Mice
MH  - Molecular Sequence Data
MH  - Pituitary Gland/metabolism
MH  - Pituitary Hormones/*genetics
MH  - Promoter Regions, Genetic
MH  - Recombinant Proteins
MH  - Transcription Factors
EDAT- 1999/12/22 00:00
MHDA- 1999/12/22 00:01
CRDT- 1999/12/22 00:00
PHST- 1999/12/22 00:00 [pubmed]
PHST- 1999/12/22 00:01 [medline]
PHST- 1999/12/22 00:00 [entrez]
AID - 10.1210/mend.13.12.0395 [doi]
PST - ppublish
SO  - Mol Endocrinol. 1999 Dec;13(12):2212-25. doi: 10.1210/mend.13.12.0395.