PMID- 10597320
OWN - NLM
STAT- MEDLINE
DCOM- 20000104
LR  - 20061115
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 18
IP  - 50
DP  - 1999 Nov 25
TI  - Search for in vivo somatic mutations in the mitotic checkpoint gene, hMAD1, in
      human lung cancers.
PG  - 7180-3
AB  - We previously reported the presence of mitotic check-point impairment in about
      40% of lung cancer cell lines. To gain an insight into the molecular basis of
      this impairment, we examined 49 lung cancer specimens for alterations in the
      hMAD1 mitotic checkpoint gene and identified a somatic, non-conservative missense
      mutation, which substitutes alanine (GCG) for threonine (ACG) at codon 299,
      together with a number of amino acid substituting, single nucleotide
      polymorphisms. This is the first demonstration of hMAD1 mutation in any type of
      human cancers. The present finding marks hMAD1 as a potential target, although
      with low frequency, for genetic alterations in lung cancer. Thus, further studies
      of hMAD1 dysfunction caused by other mechanisms appear to be warranted, as well
      as potential involvement of other components of the mitotic checkpoint.
FAU - Nomoto, S
AU  - Nomoto S
AD  - Laboratory of Ultrastructure Research, Aichi Cancer Center Research Institute,
      Nagoya, Japan.
FAU - Haruki, N
AU  - Haruki N
FAU - Takahashi, T
AU  - Takahashi T
FAU - Masuda, A
AU  - Masuda A
FAU - Koshikawa, T
AU  - Koshikawa T
FAU - Takahashi, T
AU  - Takahashi T
FAU - Fujii, Y
AU  - Fujii Y
FAU - Osada, H
AU  - Osada H
FAU - Takahashi, T
AU  - Takahashi T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (Carrier Proteins)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (DNA Primers)
RN  - 0 (MAD1L1 protein, human)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (Repressor Proteins)
SB  - IM
MH  - Base Sequence
MH  - *Carrier Proteins
MH  - Cell Cycle Proteins
MH  - DNA Primers
MH  - Humans
MH  - Lung Neoplasms/*genetics
MH  - Mitosis/*genetics
MH  - *Mutation
MH  - Nuclear Proteins/*genetics
MH  - Phosphoproteins/*genetics
MH  - *Repressor Proteins
EDAT- 1999/12/22 00:00
MHDA- 1999/12/22 00:01
CRDT- 1999/12/22 00:00
PHST- 1999/12/22 00:00 [pubmed]
PHST- 1999/12/22 00:01 [medline]
PHST- 1999/12/22 00:00 [entrez]
AID - 10.1038/sj.onc.1203141 [doi]
PST - ppublish
SO  - Oncogene. 1999 Nov 25;18(50):7180-3. doi: 10.1038/sj.onc.1203141.