PMID- 10597319
OWN - NLM
STAT- MEDLINE
DCOM- 20000104
LR  - 20171116
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 18
IP  - 50
DP  - 1999 Nov 25
TI  - Murine Siva-1 and Siva-2, alternate splice forms of the mouse Siva gene, both
      bind to CD27 but differentially transduce apoptosis.
PG  - 7174-9
AB  - CD27, a member of the TNFR family known to provide essential co-stimulatory
      signals for T cell growth and B cell Ig synthesis, can also mediate cell death.
      Using the CD27 cytoplasmic tail as the bait in yeast two hybrid assay, we
      previously cloned human Siva, a pro-apoptotic molecule. Here we report the
      characterization of the mouse Siva gene as a 4 kb sequence containing 4 exons and
      3 introns. RT-PCR has revealed the presence of two forms of mouse Siva mRNA, the 
      longer full length form Siva-1 and the shorter Siva-2 lacking the sequence coded 
      by exon 2. Immunoblotting with anti-Siva (human) antibodies clearly demonstrate
      the presence of both Siva-1 and Siva-2. Cotransfection experiments in 293T cells 
      reveal that mouse CD27 receptor can interact with both forms of Siva. Although
      mouse Siva-1 can trigger apoptosis in Rat-1 cells and in some of the mouse cell
      lines in transient transfection experiments, similar to the observation made with
      human Siva, intriguingly its alternate splice form, Siva-2 appears to be much
      less toxic. It is therefore likely that Siva-2 could regulate the function of
      Siva-1.
FAU - Yoon, Y
AU  - Yoon Y
AD  - Department of Medicine, Harvard Medical School, Boston, MA 02115, USA.
FAU - Ao, Z
AU  - Ao Z
FAU - Cheng, Y
AU  - Cheng Y
FAU - Schlossman, S F
AU  - Schlossman SF
FAU - Prasad, K V
AU  - Prasad KV
LA  - eng
SI  - GENBANK/AF033112
SI  - GENBANK/AF033113
SI  - GENBANK/AF033114
SI  - GENBANK/AF033115
GR  - GM56706/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (Carrier Proteins)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Protein Isoforms)
RN  - 0 (SIVA1 protein, human)
RN  - 0 (Siva protein, mouse)
RN  - 0 (Siva protein, rat)
RN  - 0 (Tumor Necrosis Factor Receptor Superfamily, Member 7)
SB  - IM
MH  - 3T3 Cells
MH  - *Alternative Splicing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Apoptosis/*genetics
MH  - Apoptosis Regulatory Proteins
MH  - Carrier Proteins/genetics/*metabolism
MH  - Humans
MH  - *Intracellular Signaling Peptides and Proteins
MH  - Mice
MH  - Molecular Sequence Data
MH  - Protein Binding
MH  - Protein Isoforms/genetics/*metabolism
MH  - Sequence Homology, Amino Acid
MH  - *Transduction, Genetic
MH  - Tumor Necrosis Factor Receptor Superfamily, Member 7/*metabolism
EDAT- 1999/12/22 00:00
MHDA- 1999/12/22 00:01
CRDT- 1999/12/22 00:00
PHST- 1999/12/22 00:00 [pubmed]
PHST- 1999/12/22 00:01 [medline]
PHST- 1999/12/22 00:00 [entrez]
AID - 10.1038/sj.onc.1203144 [doi]
PST - ppublish
SO  - Oncogene. 1999 Nov 25;18(50):7174-9. doi: 10.1038/sj.onc.1203144.