PMID- 10597306
OWN - NLM
STAT- MEDLINE
DCOM- 20000104
LR  - 20131121
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 18
IP  - 50
DP  - 1999 Nov 25
TI  - Characterization of the chronic myelomonocytic leukemia associated TEL-PDGF beta 
      R fusion protein.
PG  - 7055-62
AB  - The t(5;12) translocation, associated with chronic myelomonocytic leukemia,
      generates a novel gene encoding a protein, TEL-PDGF beta R, composed of the 154
      amino-terminal amino acids of the transcription factor TEL and the transmembrane 
      and intracellular part of the PDGF beta-receptor (PDGF beta R). TEL also occurs
      as a tumor-associated fusion partner for the tyrosine kinases c-ABL, JAK2 and
      TRK-C. Previous studies have demonstrated growth promoting activity of TEL-PDGF
      beta R and also indicated that the TEL moiety activates the tyrosine kinase of
      the PDGF beta R through the formation of TEL-PDGF beta R oligomers. We
      demonstrate that tyrosine phosphorylation of the fusion protein can be attenuated
      through overexpression of the TEL part of TEL-PDGF beta R, suggesting a strategy 
      for antagonizing the signaling of TEL-PDGF beta R, and other TEL-fusion proteins 
      containing tyrosine kinase domains. Comparison of BaF/3 cell lines expressing
      TEL-PDGF beta R and ligand-stimulated PDGF beta R revealed that only TEL-PDGF
      beta R expression conferred IL-3-independent growth, suggesting differences in
      signaling capacity of the two proteins. Finally, tyrosine residues 17 and 27 in
      TEL-PDGF beta R was identified as autophosphorylation sites in TEL-PDGF beta R.
FAU - Sjoblom, T
AU  - Sjoblom T
AD  - Ludwig Institute for Cancer Research, Uppsala, Sweden.
FAU - Boureux, A
AU  - Boureux A
FAU - Ronnstrand, L
AU  - Ronnstrand L
FAU - Heldin, C H
AU  - Heldin CH
FAU - Ghysdael, J
AU  - Ghysdael J
FAU - Ostman, A
AU  - Ostman A
LA  - eng
PT  - Journal Article
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (DNA Primers)
RN  - 0 (Ligands)
RN  - 0 (Oncogene Proteins, Fusion)
RN  - 0 (TEL-PDGFRbeta fusion protein, human)
RN  - 42HK56048U (Tyrosine)
RN  - 47E5O17Y3R (Phenylalanine)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - COS Cells
MH  - DNA Primers
MH  - Humans
MH  - Leukemia, Myelomonocytic, Chronic/*genetics
MH  - Ligands
MH  - Mutagenesis, Site-Directed
MH  - Oncogene Proteins, Fusion/*genetics/metabolism
MH  - Phenylalanine/genetics
MH  - Phosphorylation
MH  - Tumor Cells, Cultured
MH  - Tyrosine/genetics/metabolism
EDAT- 1999/12/22 00:00
MHDA- 1999/12/22 00:01
CRDT- 1999/12/22 00:00
PHST- 1999/12/22 00:00 [pubmed]
PHST- 1999/12/22 00:01 [medline]
PHST- 1999/12/22 00:00 [entrez]
AID - 10.1038/sj.onc.1203190 [doi]
PST - ppublish
SO  - Oncogene. 1999 Nov 25;18(50):7055-62. doi: 10.1038/sj.onc.1203190.