PMID- 10597297
OWN - NLM
STAT- MEDLINE
DCOM- 20000104
LR  - 20161124
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 18
IP  - 50
DP  - 1999 Nov 25
TI  - MKP5, a new member of the MAP kinase phosphatase family, which selectively
      dephosphorylates stress-activated kinases.
PG  - 6981-8
AB  - Dual-specificity protein tyrosine phosphatases are a burgeoning family of
      enzymes, some of which, the MKPs, are implicated in the regulation of
      mitogen-activated protein (MAP) kinases. MKPs have been shown to reverse the
      activation of the MAP kinases by hydrolyzing phosphothreonine and phosphotyrosine
      residues present in the substrates. Here we describe the characterization of a
      novel member of the MKP family, MKP5. The MKP5 gene, which maps to human
      chromosome 1q32, is expressed tissue-specifically as two transcripts of
      approximately 3.4 and 2.4 kb in human liver and skeletal muscle. When expressed
      in mammalian cells, MKP5 blocks the enzymatic activation of MAP kinases with the 
      selectivity p38 approximately JNK/SAPK >> ERK. Immunoprecipitation of endogenous 
      MAP kinases by the catalytically inactive transfected MKP5 demonstrates that it
      preferentially binds to the p38 and JNK/SAPK kinases. These findings suggest that
      the selectivity of this phosphatase may be determined at least in part at the
      level of substrate binding.
FAU - Theodosiou, A
AU  - Theodosiou A
AD  - Section of Gene Function and Regulation, Chester Beatty Laboratories, London, UK.
FAU - Smith, A
AU  - Smith A
FAU - Gillieron, C
AU  - Gillieron C
FAU - Arkinstall, S
AU  - Arkinstall S
FAU - Ashworth, A
AU  - Ashworth A
LA  - eng
SI  - GENBANK/AF179212
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (DNA, Complementary)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 3.1.3.16 (DUSP10 protein, human)
RN  - EC 3.1.3.16 (Mitogen-Activated Protein Kinase Phosphatases)
RN  - EC 3.1.3.48 (Dual-Specificity Phosphatases)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - COS Cells
MH  - DNA, Complementary
MH  - Dual-Specificity Phosphatases
MH  - Humans
MH  - Intracellular Signaling Peptides and Proteins
MH  - Mitogen-Activated Protein Kinase Phosphatases
MH  - Mitogen-Activated Protein Kinases/antagonists & inhibitors/*metabolism
MH  - Molecular Sequence Data
MH  - Phosphorylation
MH  - Protein Tyrosine Phosphatases/genetics/*metabolism
MH  - Subcellular Fractions/enzymology
MH  - Substrate Specificity
EDAT- 1999/12/22 00:00
MHDA- 1999/12/22 00:01
CRDT- 1999/12/22 00:00
PHST- 1999/12/22 00:00 [pubmed]
PHST- 1999/12/22 00:01 [medline]
PHST- 1999/12/22 00:00 [entrez]
AID - 10.1038/sj.onc.1203185 [doi]
PST - ppublish
SO  - Oncogene. 1999 Nov 25;18(50):6981-8. doi: 10.1038/sj.onc.1203185.