PMID- 10597293
OWN - NLM
STAT- MEDLINE
DCOM- 20000106
LR  - 20171116
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 18
IP  - 48
DP  - 1999 Nov 18
TI  - Covalent modification of all members of human cullin family proteins by NEDD8.
PG  - 6829-34
AB  - Recently we found that NEDD8, a ubiquitin-like protein, was linked covalently to 
      human cullin-4A (abbreviated Cul-4A) by a new ubiquitin-related pathway that is
      analogous to but distinct from the ligating system for SUMO1, another
      ubiquitin-like protein. However, it remained unknown whether the other five
      members of the family of human cullin/Cdc53 proteins are modified by NEDD8. Here 
      we report that all Hs-Cul family proteins, such as Cul-1, Cul-2, Cul-3, Cul-4B,
      and Cul-5, in addition to Cul-4A, were modified by covalent attachment of NEDD8
      in rabbit reticulocyte lysates. Moreover, by comprehensive Northern-blot
      analyses, we examined multiple tissue distributions of the messages for all
      Cul-family proteins, NEDD8, and the NEDD8-ligating system consisting of
      APP-BP1/hUba3, and hUbc12, which function as E1- and E2-like enzymes,
      respectively. The expressions of Cul-1, Cul-2, and Cul-3 resembled each other and
      were apparently correlated to those of NEDD8 and the NEDD8-ligating system in
      various human cells and tissues. However, the mRNA levels of Cul-4A, Cul-4B, and 
      Cul-5 differed considerably from each other as well as from other Cul-family
      proteins. The enhanced expression of all Cul-family proteins except Cul-5 was
      observed in a variety of tumor cell lines.
FAU - Hori, T
AU  - Hori T
AD  - Tokyo Metropolitan Institute of Medical Science, Japan.
FAU - Osaka, F
AU  - Osaka F
FAU - Chiba, T
AU  - Chiba T
FAU - Miyamoto, C
AU  - Miyamoto C
FAU - Okabayashi, K
AU  - Okabayashi K
FAU - Shimbara, N
AU  - Shimbara N
FAU - Kato, S
AU  - Kato S
FAU - Tanaka, K
AU  - Tanaka K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Cullin 1)
RN  - 0 (Cullin Proteins)
RN  - 0 (NEDD8 Protein)
RN  - 0 (NEDD8 protein, human)
RN  - 0 (RNA, Messenger)
RN  - 0 (Ubiquitins)
SB  - IM
MH  - Cell Cycle Proteins/genetics/*metabolism
MH  - *Cullin Proteins
MH  - Gene Expression Regulation
MH  - Humans
MH  - NEDD8 Protein
MH  - RNA, Messenger/genetics/metabolism
MH  - Ubiquitins/genetics/*metabolism
EDAT- 1999/12/22 00:00
MHDA- 1999/12/22 00:01
CRDT- 1999/12/22 00:00
PHST- 1999/12/22 00:00 [pubmed]
PHST- 1999/12/22 00:01 [medline]
PHST- 1999/12/22 00:00 [entrez]
AID - 10.1038/sj.onc.1203093 [doi]
PST - ppublish
SO  - Oncogene. 1999 Nov 18;18(48):6829-34. doi: 10.1038/sj.onc.1203093.