PMID- 10597252
OWN - NLM
STAT- MEDLINE
DCOM- 20000104
LR  - 20091119
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 18
IP  - 47
DP  - 1999 Nov 11
TI  - VEGI, a new member of the TNF family activates nuclear factor-kappa B and c-Jun
      N-terminal kinase and modulates cell growth.
PG  - 6496-504
AB  - Recently a new member of the human tumor necrosis factor (TNF) family named as
      VEGI was reported. However, very little is known about the biological activities 
      displayed by this cytokine. In this report, we show that in myeloid cells VEGI
      activated the transcription factor kappa B (NF-kappa B) as determined by the
      electrophoretic mobility shift assay, induced degradation of I kappa B alpha, and
      nuclear translocation of p65 subunit of NF-kappa B. VEGI also activated NF-kappa 
      B-dependent reporter gene expression. In addition, VEGI activated c-Jun
      N-terminal kinase. When examined for growth modulatory effects, VEGI inhibited
      the proliferation of breast carcinoma (MCF-7), epithelial (HeLa), and myeloid
      (U-937 and ML-1a) tumor cells; and activated caspase-3 leading to PARP cleavage. 
      VEGI-induced cytotoxicity was potentiated by inhibitors of protein synthesis.
      VEGI also induced proliferation of normal human foreskin fibroblast cells. The
      activity of VEGI could neither be neutralized by antibodies against TNF, nor
      could it compete with TNF binding, indicating that the activity of VEGI is not
      due to TNF and it binds to a distinct receptor. These results suggest that VEGI, 
      a new member of the TNF family, has a signaling pathway similar to TNF and is
      most likely a multifunctional cytokine.
FAU - Haridas, V
AU  - Haridas V
AD  - Department of Molecular Oncology, University of Texas MD Anderson Cancer Center, 
      Houston 77030, USA.
FAU - Shrivastava, A
AU  - Shrivastava A
FAU - Su, J
AU  - Su J
FAU - Yu, G L
AU  - Yu GL
FAU - Ni, J
AU  - Ni J
FAU - Liu, D
AU  - Liu D
FAU - Chen, S F
AU  - Chen SF
FAU - Ni, Y
AU  - Ni Y
FAU - Ruben, S M
AU  - Ruben SM
FAU - Gentz, R
AU  - Gentz R
FAU - Aggarwal, B B
AU  - Aggarwal BB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (DNA Primers)
RN  - 0 (NF-kappa B)
RN  - 0 (Receptors, Tumor Necrosis Factor)
RN  - 0 (Recombinant Proteins)
RN  - 0 (TNFSF15 protein, human)
RN  - 0 (Tumor Necrosis Factor Ligand Superfamily Member 15)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Base Sequence
MH  - Cell Division/*physiology
MH  - DNA Primers
MH  - Enzyme Activation
MH  - Humans
MH  - JNK Mitogen-Activated Protein Kinases
MH  - Mitogen-Activated Protein Kinases/*metabolism
MH  - NF-kappa B/*metabolism
MH  - Protein Binding
MH  - Receptors, Tumor Necrosis Factor/*metabolism
MH  - Recombinant Proteins/metabolism
MH  - Tumor Necrosis Factor Ligand Superfamily Member 15
MH  - Tumor Necrosis Factor-alpha/metabolism/*physiology
EDAT- 1999/12/22 00:00
MHDA- 1999/12/22 00:01
CRDT- 1999/12/22 00:00
PHST- 1999/12/22 00:00 [pubmed]
PHST- 1999/12/22 00:01 [medline]
PHST- 1999/12/22 00:00 [entrez]
AID - 10.1038/sj.onc.1203059 [doi]
PST - ppublish
SO  - Oncogene. 1999 Nov 11;18(47):6496-504. doi: 10.1038/sj.onc.1203059.