PMID- 10597251
OWN - NLM
STAT- MEDLINE
DCOM- 20000104
LR  - 20091119
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 18
IP  - 47
DP  - 1999 Nov 11
TI  - FMIP, a novel Fms-interacting protein, affects granulocyte/macrophage
      differentiation.
PG  - 6488-95
AB  - Hematopoietic cell growth, differentiation, and commitment to a restricted
      lineage are guided by a set of cytokines acting exclusively on cells expressing
      the corresponding cytokine receptor. The macrophage colony stimulating factor
      (M-CSF, also termed CSF-1) and its cognate receptor, the tyrosine kinase c-Fms,
      are essential for monocyte and macrophage development. The underlying molecular
      mechanism, however, is poorly understood. Here we identified a novel
      Fms-interacting protein (FMIP, MW 78 kDa) which binds transiently via its
      N-terminal 144 residues to the cytoplasmic domain of activated Fms-molecules.
      Binding of FMIP was paralleled by rapid tyrosine phosphorylation within the
      binding domain which drastically reduced its ability to associate with Fms.
      Binding was specific as evidenced by co-immunoprecipitation and association with 
      recombinant GST-Fms fusion proteins. No binding was observed with the tyrosine
      phosphorylated cytoplasmic domains of c-Kit, TrkA, c-Met, and the insulin
      receptor. The role of FMIP in hematopoietic differentiation was studied in the
      bipotential myeloid progenitor cell line, FDC-P1Mac11. Overexpression of FMIP
      prevented M-CSF induced macrophage differentiation. Instead, cells differentiated
      into granulocytes. Our data suggest that the level of FMIP expression could form 
      a threshold that decides about differentiation either into macrophages or into
      granulocytes.
FAU - Tamura, T
AU  - Tamura T
AD  - Institut fur Biochemie, Medizinische Hochschule Hannover, Germany.
FAU - Mancini, A
AU  - Mancini A
FAU - Joos, H
AU  - Joos H
FAU - Koch, A
AU  - Koch A
FAU - Hakim, C
AU  - Hakim C
FAU - Dumanski, J
AU  - Dumanski J
FAU - Weidner, K M
AU  - Weidner KM
FAU - Niemann, H
AU  - Niemann H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (Carrier Proteins)
RN  - 0 (Fmip protein, mouse)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 81627-83-0 (Macrophage Colony-Stimulating Factor)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
RN  - EC 2.7.10.1 (Receptor, Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Amino Acid Sequence
MH  - Carrier Proteins/chemistry/genetics/*metabolism
MH  - *Cell Differentiation
MH  - Cell Line
MH  - Granulocytes/*cytology
MH  - *Intracellular Signaling Peptides and Proteins
MH  - Macrophage Colony-Stimulating Factor/physiology
MH  - Macrophages/*cytology
MH  - Molecular Sequence Data
MH  - Phosphorylation
MH  - Protein Binding
MH  - Receptor Protein-Tyrosine Kinases/metabolism
MH  - Receptor, Macrophage Colony-Stimulating Factor/metabolism
MH  - Substrate Specificity
EDAT- 1999/12/22 00:00
MHDA- 1999/12/22 00:01
CRDT- 1999/12/22 00:00
PHST- 1999/12/22 00:00 [pubmed]
PHST- 1999/12/22 00:01 [medline]
PHST- 1999/12/22 00:00 [entrez]
AID - 10.1038/sj.onc.1203062 [doi]
PST - ppublish
SO  - Oncogene. 1999 Nov 11;18(47):6488-95. doi: 10.1038/sj.onc.1203062.