PMID- 10597218
OWN - NLM
STAT- MEDLINE
DCOM- 20000110
LR  - 20061115
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 18
IP  - 46
DP  - 1999 Nov 4
TI  - The generation of nfkb2 p52: mechanism and efficiency.
PG  - 6201-8
AB  - nfkb2 encodes two members of the NF-kappa B/Rel family of proteins: p52 and p100.
      The p100 polypeptide has been proposed to serve as a precursor of p52, which
      corresponds to the N-terminal half of p100. While p52 functions as a Rel
      transcription factor, the larger p100 protein acts as a cytoplasmic inhibitor of 
      select NF-kappa B/Rel transcription factor complexes. Because of their distinct
      functions, we have studied the biochemical basis for the production of these two 
      nfkb2-derived gene products. Like the p50 product of the nfkb1 gene, p52 is
      principally generated in a cotranslational manner involving proteolytic
      processing by the proteasome. The generation of p52 is dependent on a
      glycine-rich region (GRR) located upstream of the p52 C-terminus, and
      repositioning of this GRR alters the location of proteasome processing. In most
      cells, small amounts of p52 are produced relative to the levels of p100, unlike
      the usually balanced production of nfkb1-derived p50 and p105. Using p100/p105
      chimeras containing different segments of the nfkb1 and nfkb2 genes, we have
      found that diminished p52 processing is a property conferred by peptide sequences
      located downstream of the GRR, flanking the site of p52 processing.
FAU - Heusch, M
AU  - Heusch M
AD  - Gladstone Institute of Virology and Immunology, University of California, San
      Francisco 94141-9100, USA.
FAU - Lin, L
AU  - Lin L
FAU - Geleziunas, R
AU  - Geleziunas R
FAU - Greene, W C
AU  - Greene WC
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (Multienzyme Complexes)
RN  - 0 (NF-kappa B)
RN  - 0 (NF-kappa B p50 Subunit)
RN  - 0 (NF-kappa B p52 Subunit)
RN  - 0 (Protein Precursors)
RN  - 0 (Recombinant Fusion Proteins)
RN  - EC 3.4.22.- (Cysteine Endopeptidases)
RN  - EC 3.4.25.1 (Proteasome Endopeptidase Complex)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - CHO Cells
MH  - Cricetinae
MH  - Cricetulus
MH  - Cysteine Endopeptidases/drug effects/metabolism
MH  - Humans
MH  - Mice
MH  - Molecular Sequence Data
MH  - Multienzyme Complexes/drug effects/metabolism
MH  - NF-kappa B/*biosynthesis/genetics/metabolism
MH  - NF-kappa B p50 Subunit
MH  - NF-kappa B p52 Subunit
MH  - Proteasome Endopeptidase Complex
MH  - Protein Biosynthesis
MH  - Protein Precursors/genetics/*metabolism
MH  - *Protein Processing, Post-Translational
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - Transcription, Genetic
MH  - Transfection
EDAT- 1999/12/22 00:00
MHDA- 1999/12/22 00:01
CRDT- 1999/12/22 00:00
PHST- 1999/12/22 00:00 [pubmed]
PHST- 1999/12/22 00:01 [medline]
PHST- 1999/12/22 00:00 [entrez]
AID - 10.1038/sj.onc.1203022 [doi]
PST - ppublish
SO  - Oncogene. 1999 Nov 4;18(46):6201-8. doi: 10.1038/sj.onc.1203022.