PMID- 10596943
OWN - NLM
STAT- MEDLINE
DCOM- 20000119
LR  - 20121115
IS  - 0914-9465 (Print)
IS  - 0914-9465 (Linking)
VI  - 62
IP  - 4
DP  - 1999 Oct
TI  - Growth of collagen fibrils produced by human osteosarcoma cells: high-resolution 
      scanning electron microscopy.
PG  - 327-35
AB  - To demonstrate three-dimensionally the process of the collagen fibril growth, the
      bottom of culture dishes with human osteosarcoma cells (NOS-1) and their extracts
      were examined by conventional scanning electron microscopy (SEM). Backscattered
      electron (BSE) imaging of SEM was also applied to the specimens, which were
      stained with phosphotungustic acid and uranyl acetate. Conventional SEM images
      showed several stages of collagen fibril assembly. Short collagen fibrils with
      tapered ends were distributed at the bottom of the dish just beneath and/or
      around the cultured cells; they were 1 microm long and 20-30 nm in diameter at
      the thickest middle portion. These fibrils were often twisted and united in a
      right helical direction, and consequently increased in length (5-10 microm) and
      diameter (more than 100 nm). In BSE images, the periodical bands stained with
      phosphotungstic acid and uranyl acetate were visualized throughout the individual
      fibrils. The banding pattern indicated that the polarity of the collagen
      molecules was unidirectional; namely, that all molecules were pointed in the same
      direction throughout the length of the fibrils.
FAU - Hashizume, H
AU  - Hashizume H
AD  - Department of Anatomy and Histology, Niigata University School of Medicine,
      Asahimachi-dori, Japan. hasizume@med.niigata-u.ac.jp
FAU - Hitomi, J
AU  - Hitomi J
FAU - Ushiki, T
AU  - Ushiki T
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Arch Histol Cytol
JT  - Archives of histology and cytology
JID - 8806082
RN  - 0 (Biopolymers)
RN  - 0 (Organometallic Compounds)
RN  - 12067-99-1 (Phosphotungstic Acid)
RN  - 285PN2K1AO (uranyl acetate)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Biopolymers/biosynthesis
MH  - Collagen/*biosynthesis/*ultrastructure
MH  - Humans
MH  - Microscopy, Electron, Scanning
MH  - Organometallic Compounds
MH  - Osteosarcoma/*metabolism/ultrastructure
MH  - Phosphotungstic Acid
MH  - Protein Conformation
MH  - Tumor Cells, Cultured
EDAT- 1999/12/22 00:00
MHDA- 1999/12/22 00:01
CRDT- 1999/12/22 00:00
PHST- 1999/12/22 00:00 [pubmed]
PHST- 1999/12/22 00:01 [medline]
PHST- 1999/12/22 00:00 [entrez]
PST - ppublish
SO  - Arch Histol Cytol. 1999 Oct;62(4):327-35.