PMID- 10595907
OWN - NLM
STAT- MEDLINE
DCOM- 19991228
LR  - 20220310
IS  - 0002-9440 (Print)
IS  - 0002-9440 (Linking)
VI  - 155
IP  - 6
DP  - 1999 Dec
TI  - Beta-catenin mutations are frequent in human hepatocellular carcinomas associated
      with hepatitis C virus infection.
PG  - 1795-801
AB  - Hepatocellular carcinoma (HCC) is one of the most common fatal cancers worldwide.
      Hepatitis B virus and hepatitis C virus infections, exposure to aflatoxin, and
      excessive intake of alcohol have been identified as major risk factors. However, 
      the molecular mechanisms underlying their development are still poorly
      understood. Recently, beta-catenin, one of the key components of the Wnt
      signaling pathway, has been found to be mutated in about 20% of HCCs, suggesting 
      a role of the Wnt pathway in their development. In this study, we examined
      beta-catenin and APC mutations in 22 HCCs associated with HCV infection, using
      single-strand conformation polymorphism (SSCP) followed by direct DNA sequencing.
      beta-Catenin mutations were found in nine (41%) cases, but no APC mutations were 
      found. beta-Catenin immunohistochemistry revealed nuclear accumulation of
      beta-catenin protein in all nine tumors with a beta-catenin mutation and two
      additional tumors without a mutation. These results suggest that activation of
      the Wnt signaling pathway by beta-catenin mutation contributes significantly to
      the hepatocellular carcinogenesis associated with HCV infection.
FAU - Huang, H
AU  - Huang H
AD  - Unit of Molecular Pathology, International Agency for Research on Cancer (IARC), 
      Lyon, France.
FAU - Fujii, H
AU  - Fujii H
FAU - Sankila, A
AU  - Sankila A
FAU - Mahler-Araujo, B M
AU  - Mahler-Araujo BM
FAU - Matsuda, M
AU  - Matsuda M
FAU - Cathomas, G
AU  - Cathomas G
FAU - Ohgaki, H
AU  - Ohgaki H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Pathol
JT  - The American journal of pathology
JID - 0370502
RN  - 0 (Adenomatous Polyposis Coli Protein)
RN  - 0 (CTNNB1 protein, human)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Trans-Activators)
RN  - 0 (Wnt Proteins)
RN  - 0 (Zebrafish Proteins)
RN  - 0 (beta Catenin)
SB  - IM
MH  - Adenomatous Polyposis Coli Protein
MH  - Aged
MH  - Carcinoma, Hepatocellular/*genetics/metabolism/pathology/*virology
MH  - Cytoskeletal Proteins/*genetics/metabolism
MH  - Female
MH  - Genes, APC
MH  - Hepatitis C/*complications
MH  - Humans
MH  - Immunohistochemistry
MH  - Liver Neoplasms/*genetics/metabolism/pathology/*virology
MH  - Male
MH  - Middle Aged
MH  - *Mutation
MH  - Polymorphism, Genetic
MH  - Polymorphism, Single-Stranded Conformational
MH  - Proto-Oncogene Proteins
MH  - Sequence Analysis, DNA
MH  - Signal Transduction
MH  - *Trans-Activators
MH  - Wnt Proteins
MH  - *Zebrafish Proteins
MH  - beta Catenin
PMC - PMC1866943
EDAT- 1999/12/14 00:00
MHDA- 1999/12/14 00:01
CRDT- 1999/12/14 00:00
PHST- 1999/12/14 00:00 [pubmed]
PHST- 1999/12/14 00:01 [medline]
PHST- 1999/12/14 00:00 [entrez]
AID - S0002-9440(10)65496-X [pii]
AID - 10.1016/s0002-9440(10)65496-x [doi]
PST - ppublish
SO  - Am J Pathol. 1999 Dec;155(6):1795-801. doi: 10.1016/s0002-9440(10)65496-x.