PMID- 10594240
OWN - NLM
STAT- MEDLINE
DCOM- 20000113
LR  - 20190905
IS  - 0938-8990 (Print)
IS  - 0938-8990 (Linking)
VI  - 10
IP  - 12
DP  - 1999 Dec
TI  - Alternative splicing, gene localization, and binding of SH2-B to the insulin
      receptor kinase domain.
PG  - 1160-7
AB  - The SH2-B protein is an SH2-domain-containing molecule that interacts with a
      number of phosphorylated kinase and receptor molecules including the insulin
      receptor. Two isoforms of the SH2-B have been identified and have been proposed
      to arise through alternate splicing. Here we have identified a third isoform of
      the SH2-B protein, SH2-Bgamma, that interacts specifically with the insulin
      receptor. This interaction required phosphorylation of residue Y1146 in the
      triple tyrosine motif within the activation loop of the IR kinase and is one of
      only two signaling molecules shown to interact directly with this residue of the 
      insulin receptor kinase domain. The intron/exon structure of the SH2-B gene was
      determined. Alternate splice sites utilized to generate the different isoforms of
      the SH2-B protein were identified in the 3' end of the SH2-B gene immediately
      downstream of the exon encoding the core of the SH2 domain. Additionally, the
      chromosomal location of the SH2-B gene was determined to be the distal arm of
      mouse Chromosome (Chr) 7 in a region linked to obesity in mice.
FAU - Nelms, K
AU  - Nelms K
AD  - Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, 
      National Institutes of Health, Bethesda, Maryland 20892, USA.
FAU - O'Neill, T J
AU  - O'Neill TJ
FAU - Li, S
AU  - Li S
FAU - Hubbard, S R
AU  - Hubbard SR
FAU - Gustafson, T A
AU  - Gustafson TA
FAU - Paul, W E
AU  - Paul WE
LA  - eng
SI  - GENBANK/AF074329
GR  - DK50602/DK/NIDDK NIH HHS/United States
GR  - DK52916/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mamm Genome
JT  - Mammalian genome : official journal of the International Mammalian Genome Society
JID - 9100916
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Carrier Proteins)
RN  - 0 (Protein Isoforms)
RN  - 0 (RNA, Messenger)
RN  - 0 (Sh2bpsm1 protein, mouse)
RN  - 21820-51-9 (Phosphotyrosine)
RN  - EC 2.7.10.1 (Receptor, Insulin)
SB  - IM
MH  - *Adaptor Proteins, Signal Transducing
MH  - Alternative Splicing/*genetics
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Carrier Proteins/chemistry/*genetics/*metabolism
MH  - *Chromosome Mapping
MH  - Cloning, Molecular
MH  - Exons/genetics
MH  - Introns/genetics
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - Phosphorylation
MH  - Phosphotyrosine/genetics/metabolism
MH  - Protein Binding
MH  - Protein Isoforms/chemistry/genetics/metabolism
MH  - RNA, Messenger/analysis/genetics
MH  - Receptor, Insulin/chemistry/genetics/*metabolism
MH  - Two-Hybrid System Techniques
MH  - src Homology Domains/genetics
EDAT- 1999/12/14 00:00
MHDA- 1999/12/14 00:01
CRDT- 1999/12/14 00:00
PHST- 1999/12/14 00:00 [pubmed]
PHST- 1999/12/14 00:01 [medline]
PHST- 1999/12/14 00:00 [entrez]
AID - MGS2171 [pii]
AID - 10.1007/s003359901183 [doi]
PST - ppublish
SO  - Mamm Genome. 1999 Dec;10(12):1160-7. doi: 10.1007/s003359901183.