PMID- 10594058
OWN - NLM
STAT- MEDLINE
DCOM- 20000106
LR  - 20191210
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Linking)
VI  - 19
IP  - 24
DP  - 1999 Dec 15
TI  - Protein 4.1N binding to nuclear mitotic apparatus protein in PC12 cells mediates 
      the antiproliferative actions of nerve growth factor.
PG  - 10747-56
AB  - Protein 4.1N is a neuronal selective isoform of the erythrocyte membrane
      cytoskeleton protein 4.1R. In the present study, we demonstrate an interaction
      between 4.1N and nuclear mitotic apparatus protein (NuMA), a nuclear protein
      required for mitosis. The binding involves the C-terminal domain of 4.1N. In PC12
      cells treatment with nerve growth factor (NGF) elicits translocation of 4. 1N to 
      the nucleus and promotes its association with NuMA. Specific targeting of 4.1N to
      the nucleus arrests PC12 cells at the G1 phase and produces an aberrant nuclear
      morphology. Inhibition of 4.1N nuclear translocation prevents the NGF-mediated
      arrest of cell division, which can be reversed by overexpression of 4.1N. Thus,
      nuclear 4.1N appears to mediate the antiproliferative actions of NGF by
      antagonizing the role of NuMA in mitosis.
FAU - Ye, K
AU  - Ye K
AD  - Departments of Neuroscience, Johns Hopkins University School of Medicine,
      Baltimore, Maryland 21205, USA.
FAU - Compton, D A
AU  - Compton DA
FAU - Lai, M M
AU  - Lai MM
FAU - Walensky, L D
AU  - Walensky LD
FAU - Snyder, S H
AU  - Snyder SH
LA  - eng
GR  - DA-00074/DA/NIDA NIH HHS/United States
GR  - GM-07309/GM/NIGMS NIH HHS/United States
GR  - MH18501/MH/NIMH NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for
      Neuroscience
JID - 8102140
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Neuropeptides)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Numa1 protein, rat)
RN  - 0 (erythrocyte membrane protein band 4.1-like 1)
RN  - 9061-61-4 (Nerve Growth Factor)
SB  - IM
MH  - Animals
MH  - Biological Transport/drug effects/physiology
MH  - Cell Cycle Proteins
MH  - Cell Division/drug effects/physiology
MH  - Cell Nucleus/metabolism
MH  - Cytoskeletal Proteins/*metabolism
MH  - G1 Phase/physiology
MH  - *Membrane Proteins
MH  - Nerve Growth Factor/antagonists & inhibitors/*pharmacology
MH  - Neuropeptides/*metabolism
MH  - Nuclear Proteins/*metabolism
MH  - PC12 Cells/*metabolism/pathology
MH  - Rats
PMC - PMC6784956
EDAT- 1999/12/14 00:00
MHDA- 1999/12/14 00:01
CRDT- 1999/12/14 00:00
PHST- 1999/12/14 00:00 [pubmed]
PHST- 1999/12/14 00:01 [medline]
PHST- 1999/12/14 00:00 [entrez]
PST - ppublish
SO  - J Neurosci. 1999 Dec 15;19(24):10747-56.