PMID- 10593960
OWN - NLM
STAT- MEDLINE
DCOM- 20000127
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 51
DP  - 1999 Dec 17
TI  - Identification and characterization of cvHsp. A novel human small stress protein 
      selectively expressed in cardiovascular and insulin-sensitive tissues.
PG  - 36592-600
AB  - Starting with computational tools that search for tissue-selective expression of 
      assembled expressed sequenced tags, we have identified by focusing on heart
      libraries a novel small stress protein of 170 amino acids that we named cvHsp.
      cvHsp was found as being computationally selectively and highly (0.3% of the
      total RNA) expressed in human heart. The cvHsp gene mapped to 1p36.23-p34.3
      between markers D1S434 and D1S507. The expression of cvHsp was analyzed with RNA 
      dot, Northern blots, or reverse transcription-polymerase chain reaction:
      expression was high in heart, medium in skeletal muscle, and low in aorta or
      adipose tissues. In the heart of rat models of cardiac pathologies, cvHsp mRNA
      expression was either unchanged (spontaneous hypertension), up-regulated (right
      ventricular hypertrophy induced by monocrotaline treatment), or down-regulated
      (left ventricular hypertrophy following aortic banding). In obese Zucker rats,
      cvHsp mRNA was increased in skeletal muscle, brown, and white adipose tissues but
      remained unchanged in the heart. Western blot analysis using antipeptide
      polyclonal antibodies revealed two specific bands at 23 and 25 kDa for cvHsp in
      human heart. cvHsp interacted in both yeast two-hybrid and immunoprecipitation
      experiments with alpha-filamin or actin-binding protein 280. Within cvHsp, amino 
      acid residues 56-119 were shown to be important for its specific interaction with
      the C-terminal tail of alpha-filamin.
FAU - Krief, S
AU  - Krief S
AD  - SmithKline Beecham Laboratoires Pharmaceutiques, 4 rue du Chesnay-Beauregard, BP 
      58, 35762 Saint-Gregoire, France. Stephane_Krief@sbphrd.com
FAU - Faivre, J F
AU  - Faivre JF
FAU - Robert, P
AU  - Robert P
FAU - Le Douarin, B
AU  - Le Douarin B
FAU - Brument-Larignon, N
AU  - Brument-Larignon N
FAU - Lefrere, I
AU  - Lefrere I
FAU - Bouzyk, M M
AU  - Bouzyk MM
FAU - Anderson, K M
AU  - Anderson KM
FAU - Greller, L D
AU  - Greller LD
FAU - Tobin, F L
AU  - Tobin FL
FAU - Souchet, M
AU  - Souchet M
FAU - Bril, A
AU  - Bril A
LA  - eng
SI  - GENBANK/AF155908
SI  - GENBANK/AF155909
SI  - GENBANK/AF155910
SI  - GENBANK/AJ243191
SI  - GENBANK/AJ243192
SI  - GENBANK/AJ243193
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA, Complementary)
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (Insulin)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cardiovascular System/*metabolism
MH  - Cloning, Molecular
MH  - DNA, Complementary/genetics/isolation & purification
MH  - Gene Expression Regulation
MH  - Heat-Shock Proteins/*biosynthesis/*genetics
MH  - Humans
MH  - Insulin/*metabolism
MH  - Molecular Sequence Data
MH  - Organ Specificity
MH  - Rats
MH  - Sequence Alignment
EDAT- 1999/12/14 00:00
MHDA- 1999/12/14 00:01
CRDT- 1999/12/14 00:00
PHST- 1999/12/14 00:00 [pubmed]
PHST- 1999/12/14 00:01 [medline]
PHST- 1999/12/14 00:00 [entrez]
AID - 10.1074/jbc.274.51.36592 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Dec 17;274(51):36592-600. doi: 10.1074/jbc.274.51.36592.