PMID- 10593953
OWN - NLM
STAT- MEDLINE
DCOM- 20000127
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 51
DP  - 1999 Dec 17
TI  - HRad17 colocalizes with NHP2L1 in the nucleolus and redistributes after UV
      irradiation.
PG  - 36544-9
AB  - The rad17 gene of Schizosaccharomyces pombe plays an important role as a
      checkpoint protein following DNA damage and during DNA replication. The human
      homologue of S. pombe rad17, Hrad17, was recently identified, but its function
      has not yet been established. Using the yeast two-hybrid system, we determined
      that HRad17 can interact with a nucleolar protein, NHP2L1. This interaction was
      also demonstrated biochemically, in human cells. Immunofluorescence studies
      revealed that HRad17 and NHP2L1 colocalize to the nucleolus, and immunogold
      labeling further resolved the location of NHP2L1 to the dense fibrillar component
      of the nucleolus. Interestingly, the localization of HRad17 in the nucleolus was 
      altered in response to UV irradiation. These results provide some insight into
      the DNA damage and replication checkpoint mechanisms of HRad17.
FAU - Chang, M S
AU  - Chang MS
AD  - Department of Cancer Biology, Dana-Farber Cancer Institute and Harvard Medical
      School, Boston, Massachusetts 02115, USA.
FAU - Sasaki, H
AU  - Sasaki H
FAU - Campbell, M S
AU  - Campbell MS
FAU - Kraeft, S K
AU  - Kraeft SK
FAU - Sutherland, R
AU  - Sutherland R
FAU - Yang, C Y
AU  - Yang CY
FAU - Liu, Y
AU  - Liu Y
FAU - Auclair, D
AU  - Auclair D
FAU - Hao, L
AU  - Hao L
FAU - Sonoda, H
AU  - Sonoda H
FAU - Ferland, L H
AU  - Ferland LH
FAU - Chen, L B
AU  - Chen LB
LA  - eng
GR  - CA38493-12/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Fungal Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Rad17 protein, human)
RN  - 0 (Ribonucleoproteins, Small Nuclear)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 136111-26-7 (NHP2 protein, S cerevisiae)
SB  - IM
MH  - Amino Acid Sequence
MH  - Cell Cycle Proteins/*genetics/metabolism
MH  - Cell Nucleolus/*genetics/metabolism
MH  - DNA Damage/radiation effects
MH  - Fungal Proteins/*genetics/metabolism
MH  - Gene Expression Regulation, Fungal/*radiation effects
MH  - Genes, Fungal
MH  - Humans
MH  - Molecular Sequence Data
MH  - Nuclear Proteins/*genetics/metabolism
MH  - *Ribonucleoproteins, Small Nuclear
MH  - *Saccharomyces cerevisiae Proteins
MH  - Schizosaccharomyces/*genetics/metabolism/ultrastructure
MH  - Sequence Alignment
MH  - Ultraviolet Rays
EDAT- 1999/12/14 00:00
MHDA- 1999/12/14 00:01
CRDT- 1999/12/14 00:00
PHST- 1999/12/14 00:00 [pubmed]
PHST- 1999/12/14 00:01 [medline]
PHST- 1999/12/14 00:00 [entrez]
AID - 10.1074/jbc.274.51.36544 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Dec 17;274(51):36544-9. doi: 10.1074/jbc.274.51.36544.