PMID- 10593949
OWN - NLM
STAT- MEDLINE
DCOM- 20000127
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 51
DP  - 1999 Dec 17
TI  - Association of two nuclear proteins, Npw38 and NpwBP, via the interaction between
      the WW domain and a novel proline-rich motif containing glycine and arginine.
PG  - 36513-9
AB  - We have previously reported a nuclear protein possessing a WW domain, Npw38
      (Komuro, A., Saeki, M., and Kato, S. (1999) Nucleic Acids Res. 27, 1957-1965).
      Here we report a Npw38-binding protein, NpwBP, isolated from HeLa cell nuclear
      extracts and its characterization using a cloned cDNA. NpwBP contains two
      proline-rich regions that are capable of binding to the WW domain of Npw38. The
      binding analysis using an oligopeptide-immobilized membrane revealed that the WW 
      domain of Npw38 preferentially recognizes a short proline-rich sequence, PPGPPP, 
      surrounded by an arginine residue, so we named it a PGR motif. Localization
      analysis using green fluorescent protein fusion protein and immunostaining showed
      that Npw38 and NpwBP are colocalized in the same subnuclear region.
      Coimmunoprecipitation experiments confirmed the association between Npw38 and
      NpwBP, which were expressed as epitope-tagged forms in COS7 cells. Furthermore,
      the N-terminal region of NpwBP has binding ability for poly(rG) and G-rich
      single-stranded DNA. These results suggest that NpwBP is a physiological ligand
      of Npw38 and that the Npw38-NpwBP complex may function as a component of an mRNA 
      factory in the nucleus.
FAU - Komuro, A
AU  - Komuro A
AD  - Kato Cytoprotein Network Project, ERATO, Japan Science and Technology Corporation
      (JST), c/o Sagami Chemical Research Center, Nishi-Ohnuma 4-4-1, Sagamihara,
      Kanagawa 229-0012, Japan.
FAU - Saeki, M
AU  - Saeki M
FAU - Kato, S
AU  - Kato S
LA  - eng
SI  - GENBANK/AB029309
PT  - Journal Article
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Carrier Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (PQBP1 protein, human)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (nuclear protein wBP)
RN  - 94ZLA3W45F (Arginine)
RN  - 9DLQ4CIU6V (Proline)
RN  - TE7660XO1C (Glycine)
SB  - IM
MH  - Amino Acid Sequence
MH  - Arginine
MH  - Base Sequence
MH  - Binding Sites
MH  - *Carrier Proteins
MH  - Glycine
MH  - HeLa Cells
MH  - Humans
MH  - Molecular Sequence Data
MH  - Nuclear Proteins/chemistry/*metabolism
MH  - Proline
MH  - Protein Binding
MH  - Recombinant Fusion Proteins/chemistry/metabolism
EDAT- 1999/12/14 00:00
MHDA- 1999/12/14 00:01
CRDT- 1999/12/14 00:00
PHST- 1999/12/14 00:00 [pubmed]
PHST- 1999/12/14 00:01 [medline]
PHST- 1999/12/14 00:00 [entrez]
AID - 10.1074/jbc.274.51.36513 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Dec 17;274(51):36513-9. doi: 10.1074/jbc.274.51.36513.