PMID- 10593942
OWN - NLM
STAT- MEDLINE
DCOM- 20000127
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 51
DP  - 1999 Dec 17
TI  - Molecular cloning of a zinc finger autoantigen transiently associated with
      interphase nucleolus and mitotic centromeres and midbodies. Orthologous proteins 
      with nine CXXC motifs highly conserved from nematodes to humans.
PG  - 36456-64
AB  - We have cloned a novel human autoimmune antigen in a patient suffering from
      rheumatoid arthritis with high levels of antibodies to the nucleolus organizer
      regions. Initially the human autoimmune serum was used to select a cDNA of 317
      amino acids from a hamster expression library. Using the hamster DNA as a probe, 
      we isolated the human homologous cDNA of 320 amino acids. Human and hamster
      polypeptides share a 95% amino acid homology. The deduced 36-kDa protein contains
      a putative amino-terminal NLS signal, nine cysteine-X-X-cysteine motifs highly
      conserved, and a carboxyl-terminal poly acidic region. Several homologous
      expressed sequence tags have been identified in data bases suggesting that
      orthologous proteins are present throughout evolution from worms to humans. A
      Drosophila expressed sequence tag was further completely sequenced for a
      full-length protein with 60% amino acid identity to the human homologue. Northern
      blot analysis revealed that this novel protein is widely distributed in human
      tissues with significantly higher expression levels in heart and skeletal muscle.
      Specific antibodies to the recombinant protein and transfection experiments
      demonstrated by immunofluorescence the localization of the protein predominantly 
      but not exclusively to the nucleolus of interphase mammalian cells. In
      actinomycin D-treated cells the protein remains associated with the nucleolus but
      is not segregated, like other ribosomal factors such as upstream binding factor. 
      In mitosis the protein was found to be associated with centromeres and
      concentrated at the midbody in cytokinesis. Transient distribution of this
      evolutionarily conserved zinc finger nucleolar autoantigen to the mitotic
      centromeres may provide the means for several aspects of cell cycle control and
      transcriptional regulation.
FAU - Bolivar, J
AU  - Bolivar J
AD  - Departamento de Bioquimica y Biologia Molecular, Facultad de Ciencias,
      Universidad de Cadiz, 11510 Puerto Real, Cadiz, Spain.
FAU - Diaz, I
AU  - Diaz I
FAU - Iglesias, C
AU  - Iglesias C
FAU - Valdivia, M M
AU  - Valdivia MM
LA  - eng
SI  - GENBANK/AJ006591
SI  - GENBANK/AJ131564
SI  - GENBANK/Y12836
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Autoantigens)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Autoantigens/biosynthesis/*genetics/immunology
MH  - Base Sequence
MH  - Cell Nucleolus/*genetics/immunology/metabolism
MH  - Centromere/*genetics/immunology/metabolism
MH  - Cloning, Molecular
MH  - Cricetinae
MH  - Gene Expression Regulation/immunology
MH  - Humans
MH  - Interphase/*genetics
MH  - Mitosis/*genetics
MH  - Molecular Sequence Data
MH  - Nematoda
MH  - Sequence Alignment
MH  - Sequence Analysis
MH  - Zinc Fingers
EDAT- 1999/12/14 00:00
MHDA- 1999/12/14 00:01
CRDT- 1999/12/14 00:00
PHST- 1999/12/14 00:00 [pubmed]
PHST- 1999/12/14 00:01 [medline]
PHST- 1999/12/14 00:00 [entrez]
AID - 10.1074/jbc.274.51.36456 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Dec 17;274(51):36456-64. doi: 10.1074/jbc.274.51.36456.