PMID- 10593918
OWN - NLM
STAT- MEDLINE
DCOM- 20000127
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 51
DP  - 1999 Dec 17
TI  - MIR is a novel ERM-like protein that interacts with myosin regulatory light chain
      and inhibits neurite outgrowth.
PG  - 36288-92
AB  - The ERM protein family members ezrin, radixin, and moesin are cytoskeletal
      effector proteins linking actin to membrane-bound proteins at the cell surface.
      Here we report on the cloning of myosin regulatory light chain interacting
      protein (MIR), a protein with an ERM-homology domain and a carboxyl-terminal RING
      finger, that is expressed, among other tissues, in brain. MIR is distributed in
      cultured COS cells, in a punctuated manner as shown using enhanced green
      fluorescent protein (EGFP)-tagged MIR and by staining with a specific antibody
      for MIR. In the yeast two-hybrid system and in transfected COS cells, MIR
      interacts with myosin regulatory light chain B, which in turn regulates the
      activity of the actomyosin complex. Overexpression of MIR cDNA in PC12 cells
      abrogated neurite outgrowth induced by nerve growth factor (NGF) without
      affecting TrkA signaling. The results show that MIR, a novel ERM-like protein,
      affects cytoskeleton interactions regulating cell motility, such as neurite
      outgrowth.
FAU - Olsson, P A
AU  - Olsson PA
AD  - Department of Neuroscience, Neurobiology, Uppsala University, Box 587 Biomedical 
      Center, S-75123 Uppsala, Sweden.
FAU - Korhonen, L
AU  - Korhonen L
FAU - Mercer, E A
AU  - Mercer EA
FAU - Lindholm, D
AU  - Lindholm D
LA  - eng
SI  - GENBANK/AF187016
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Myosin Light Chains)
RN  - 0 (Nerve Tissue Proteins)
RN  - EC 2.3.2.27 (MYLIP protein, human)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Carrier Proteins/*genetics
MH  - Cell Differentiation/physiology
MH  - Cloning, Molecular
MH  - DNA, Complementary/genetics/isolation & purification
MH  - Humans
MH  - Molecular Sequence Data
MH  - Myosin Light Chains/*physiology
MH  - Nerve Tissue Proteins/*physiology
MH  - Neurites/*physiology/ultrastructure
MH  - Neurons/*physiology/ultrastructure
MH  - PC12 Cells
MH  - Rats
MH  - Sequence Alignment
MH  - Ubiquitin-Protein Ligases
EDAT- 1999/12/14 00:00
MHDA- 1999/12/14 00:01
CRDT- 1999/12/14 00:00
PHST- 1999/12/14 00:00 [pubmed]
PHST- 1999/12/14 00:01 [medline]
PHST- 1999/12/14 00:00 [entrez]
AID - 10.1074/jbc.274.51.36288 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Dec 17;274(51):36288-92. doi: 10.1074/jbc.274.51.36288.