PMID- 10593899
OWN - NLM
STAT- MEDLINE
DCOM- 20000127
LR  - 20190508
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 274
IP  - 51
DP  - 1999 Dec 17
TI  - The leucine-based sorting motifs in the cytoplasmic domain of the invariant chain
      are recognized by the clathrin adaptors AP1 and AP2 and their medium chains.
PG  - 36153-8
AB  - Recognition of sorting signals within the cytoplasmic tail of membrane proteins
      by adaptor protein complexes is a crucial step in membrane protein sorting. The
      three known adaptor complexes, AP1, AP2, and AP3, have all been shown to
      recognize tyrosine- and leucine-based sorting signals, which are the most common 
      sorting signals within membrane protein cytoplasmic tails. Although
      tyrosine-based signals are recognized by the micro-chains of adaptor complexes,
      the subunit recognizing leucine-based sorting signals is less clear. In this
      report we show by surface plasmon resonance that the two leucine-based sorting
      signals within the cytoplasmic tail of the invariant chain bind independently
      from each other to AP1 and AP2 but not to AP3. We also show that both motifs can 
      be recognized by the micro-chains of AP1 and AP2. Moreover, by using monomeric as
      well as trimeric invariant chain constructs, we show that adaptor binding does
      not require trimerization of the invariant chain.
FAU - Hofmann, M W
AU  - Hofmann MW
AD  - Center for Molecular Biology, University of Heidelberg, 69120 Heidelberg,
      Germany.
FAU - Honing, S
AU  - Honing S
FAU - Rodionov, D
AU  - Rodionov D
FAU - Dobberstein, B
AU  - Dobberstein B
FAU - von Figura, K
AU  - von Figura K
FAU - Bakke, O
AU  - Bakke O
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Adaptor Protein Complex 1)
RN  - 0 (Adaptor Protein Complex 2)
RN  - 0 (Adaptor Protein Complex 3)
RN  - 0 (Adaptor Protein Complex alpha Subunits)
RN  - 0 (Adaptor Proteins, Vesicular Transport)
RN  - 0 (Clathrin)
RN  - 0 (Membrane Proteins)
RN  - 0 (Monomeric Clathrin Assembly Proteins)
RN  - 0 (clathrin assembly protein AP180)
RN  - GMW67QNF9C (Leucine)
SB  - IM
MH  - Adaptor Protein Complex 1
MH  - Adaptor Protein Complex 2
MH  - Adaptor Protein Complex 3
MH  - Adaptor Protein Complex alpha Subunits
MH  - Adaptor Proteins, Vesicular Transport
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cell Line
MH  - Clathrin/chemistry/metabolism
MH  - Leucine
MH  - Membrane Proteins/*chemistry/metabolism
MH  - Mice
MH  - Molecular Sequence Data
MH  - *Monomeric Clathrin Assembly Proteins
MH  - Rats
EDAT- 1999/12/14 00:00
MHDA- 1999/12/14 00:01
CRDT- 1999/12/14 00:00
PHST- 1999/12/14 00:00 [pubmed]
PHST- 1999/12/14 00:01 [medline]
PHST- 1999/12/14 00:00 [entrez]
AID - 10.1074/jbc.274.51.36153 [doi]
PST - ppublish
SO  - J Biol Chem. 1999 Dec 17;274(51):36153-8. doi: 10.1074/jbc.274.51.36153.