PMID- 10593653
OWN - NLM
STAT- MEDLINE
DCOM- 20000103
LR  - 20131121
IS  - 1044-9523 (Print)
IS  - 1044-9523 (Linking)
VI  - 10
IP  - 11
DP  - 1999 Nov
TI  - BUBR1 phosphorylation is regulated during mitotic checkpoint activation.
PG  - 769-75
AB  - Eukaryotic cells have evolved a mechanism that delays the progression of mitosis 
      until condensed chromosomes are properly positioned on the mitotic spindle. To
      understand the molecular basis of such monitoring mechanism in human cells, we
      have been studying genes that regulate the mitotic checkpoint. Our early studies 
      have led to the cloning of a full-length cDNA encoding MAD3-like protein (also
      termed BUBR1/MAD3/SSK1). Dot blot analyses show that BUBR1 mRNA is expressed in
      tissues with a high mitotic index but not in differentiated tissues. Western blot
      analyses show that in asynchronous cells, BUBR1 protein primarily exhibits a
      molecular mass of 120 kDa, and its expression is detected in most cell lines
      examined. In addition, BUBR1 is present during various stages of the cell cycle. 
      As cells enter later S and G2, BUBR1 levels are increased significantly.
      Nocodazole-arrested mitotic cells obtained by mechanical shake-off contain BUBR1 
      antigen with a slower mobility on denaturing SDS gels. Phosphatase treatment
      restores the slowly migrating band to the interphase state, indicating that the
      slow mobility of the BUBR1 antigen is attributable to phosphorylation.
      Furthermore, purified recombinant His6-BUBR1 is capable of autophosphorylation.
      Our studies indicate that BUBR1 phosphorylation status is regulated during
      spindle disruption. Considering its strong homology to BUB1 protein kinase, BUBR1
      may also play an important role in mitotic checkpoint control by phosphorylation 
      of a critical cellular component(s) of the mitotic checkpoint pathway.
FAU - Li, W
AU  - Li W
AD  - Department of Internal Medicine, University of Cincinnati College of Medicine,
      Ohio 45267, USA.
FAU - Lan, Z
AU  - Lan Z
FAU - Wu, H
AU  - Wu H
FAU - Wu, S
AU  - Wu S
FAU - Meadows, J
AU  - Meadows J
FAU - Chen, J
AU  - Chen J
FAU - Zhu, V
AU  - Zhu V
FAU - Dai, W
AU  - Dai W
LA  - eng
GR  - CA74299/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cell Growth Differ
JT  - Cell growth & differentiation : the molecular biology journal of the American
      Association for Cancer Research
JID - 9100024
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.11.1 (BUB1 protein, human)
RN  - EC 2.7.11.1 (Bub1 spindle checkpoint protein)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
SB  - IM
MH  - Cell Cycle Proteins/genetics/*metabolism
MH  - DNA, Complementary
MH  - G1 Phase/physiology
MH  - Gene Expression Regulation, Enzymologic
MH  - Gene Expression Regulation, Neoplastic
MH  - HeLa Cells
MH  - Humans
MH  - Mitosis/*physiology
MH  - Phosphorylation
MH  - Protein Kinases/genetics/*metabolism
MH  - Protein-Serine-Threonine Kinases
MH  - RNA, Messenger/analysis
MH  - Transfection
EDAT- 1999/12/11 00:00
MHDA- 1999/12/11 00:01
CRDT- 1999/12/11 00:00
PHST- 1999/12/11 00:00 [pubmed]
PHST- 1999/12/11 00:01 [medline]
PHST- 1999/12/11 00:00 [entrez]
PST - ppublish
SO  - Cell Growth Differ. 1999 Nov;10(11):769-75.