PMID- 10591186
OWN - NLM
STAT- MEDLINE
DCOM- 20000104
LR  - 20191210
IS  - 1074-7613 (Print)
IS  - 1074-7613 (Linking)
VI  - 11
IP  - 5
DP  - 1999 Nov
TI  - Physical and functional association of LFA-1 with DNAM-1 adhesion molecule.
PG  - 615-23
AB  - Whereas ligation of the DNAM-1 adhesion molecule triggers cytotoxicity mediated
      by normal NK and T cells, this function was defective in NK cell clones from
      leukocyte adhesion deficiency syndrome. However, genetic reconstitution of cell
      surface expression of LFA-1 restored the ability of DNAM-1 to initiate
      anti-DNAM-1 mAb-induced cytotoxicity, indicating a functional relationship
      between DNAM-1 and LFA-1. Further studies demonstrated that LFA-1 physically
      associates with DNAM-1 in NK cells and anti-CD3 mAb stimulated T cells, for which
      serine phosphorylation of DNAM-1 plays a critical role. In addition,
      cross-linking of LFA-1 induces tyrosine phosphorylation of DNAM-1, for which the 
      Fyn protein tyrosine kinase is responsible. These results indicate that DNAM-1 is
      involved in the LFA-1-mediated intracellular signals.
FAU - Shibuya, K
AU  - Shibuya K
AD  - Department of Immunology, Institute of Basic Medical Sciences, Center for TARA,
      University of Tsukuba, Japan.
FAU - Lanier, L L
AU  - Lanier LL
FAU - Phillips, J H
AU  - Phillips JH
FAU - Ochs, H D
AU  - Ochs HD
FAU - Shimizu, K
AU  - Shimizu K
FAU - Nakayama, E
AU  - Nakayama E
FAU - Nakauchi, H
AU  - Nakauchi H
FAU - Shibuya, A
AU  - Shibuya A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Immunity
JT  - Immunity
JID - 9432918
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Differentiation, T-Lymphocyte)
RN  - 0 (CD226 antigen)
RN  - 0 (CD3 Complex)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Lymphocyte Function-Associated Antigen-1)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 17885-08-4 (Phosphoserine)
RN  - EC 2.7.10.2 (FYN protein, human)
RN  - EC 2.7.10.2 (Fyn protein, mouse)
RN  - EC 2.7.10.2 (Proto-Oncogene Proteins c-fyn)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/immunology
MH  - *Antigens, Differentiation, T-Lymphocyte
MH  - CD3 Complex/immunology
MH  - COS Cells
MH  - Cell Adhesion
MH  - Cell Adhesion Molecules/chemistry/immunology/*physiology
MH  - Chlorocebus aethiops
MH  - Cytotoxicity, Immunologic
MH  - Genetic Complementation Test
MH  - Humans
MH  - Jurkat Cells
MH  - Killer Cells, Natural/immunology
MH  - Leukocyte-Adhesion Deficiency Syndrome/immunology/*pathology
MH  - Lymphocyte Activation
MH  - Lymphocyte Function-Associated Antigen-1/chemistry/*physiology
MH  - Mice
MH  - Phosphorylation
MH  - Phosphoserine/metabolism
MH  - Protein Processing, Post-Translational
MH  - Proto-Oncogene Proteins/physiology
MH  - Proto-Oncogene Proteins c-fyn
MH  - Signal Transduction/*physiology
MH  - T-Lymphocytes/immunology
MH  - Tumor Cells, Cultured
EDAT- 1999/12/11 00:00
MHDA- 1999/12/11 00:01
CRDT- 1999/12/11 00:00
PHST- 1999/12/11 00:00 [pubmed]
PHST- 1999/12/11 00:01 [medline]
PHST- 1999/12/11 00:00 [entrez]
AID - S1074-7613(00)80136-3 [pii]
AID - 10.1016/s1074-7613(00)80136-3 [doi]
PST - ppublish
SO  - Immunity. 1999 Nov;11(5):615-23. doi: 10.1016/s1074-7613(00)80136-3.