PMID- 10591182
OWN - NLM
STAT- MEDLINE
DCOM- 20000104
LR  - 20190915
IS  - 1074-7613 (Print)
IS  - 1074-7613 (Linking)
VI  - 11
IP  - 5
DP  - 1999 Nov
TI  - Identification of podocalyxin-like protein 1 as a novel cell surface marker for
      hemangioblasts in the murine aorta-gonad-mesonephros region.
PG  - 567-78
AB  - Recent studies with avian embryos and murine embryonic stem cells have suggested 
      that hematopoietic cells are derived from hemangioblasts, the common precursors
      of hematopoietic and endothelial cells. We molecularly cloned podocalyxin-like
      protein 1 (PCLP1) as a novel surface marker for endothelial-like cells in the
      aorta-gonad-mesonephros (AGM) region of mouse embryos, where long-term
      repopulating hematopoietic stem cells (LTR-HSCs) are known to arise. PCLP1+ CD45 
      cells in the AGM region incorporated acetylated low-density lipoprotein and
      produced both hematopoietic and endothelial cells when cocultured with OP9
      stromal cells. Moreover, multiple lineages of hematopoietic cells were generated 
      in vivo when PCLP1 +CD45-cells were injected into neonatal liver of
      busulfan-treated mice. Thus, PCLP1 can be used to separate hemangioblasts that
      give rise to LTR-HSCs.
FAU - Hara, T
AU  - Hara T
AD  - Institute of Molecular and Cellular Biosciences, The University of Tokyo, Japan. 
      thara@rinshoken.or.jp
FAU - Nakano, Y
AU  - Nakano Y
FAU - Tanaka, M
AU  - Tanaka M
FAU - Tamura, K
AU  - Tamura K
FAU - Sekiguchi, T
AU  - Sekiguchi T
FAU - Minehata, K
AU  - Minehata K
FAU - Copeland, N G
AU  - Copeland NG
FAU - Jenkins, N A
AU  - Jenkins NA
FAU - Okabe, M
AU  - Okabe M
FAU - Kogo, H
AU  - Kogo H
FAU - Mukouyama, Y
AU  - Mukouyama Y
FAU - Miyajima, A
AU  - Miyajima A
LA  - eng
SI  - GENBANK/AB028048
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Immunity
JT  - Immunity
JID - 9432918
RN  - 0 (Biomarkers)
RN  - 0 (Fetal Proteins)
RN  - 0 (Growth Substances)
RN  - 0 (Lipoproteins, LDL)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Osm protein, mouse)
RN  - 0 (Peptides)
RN  - 0 (acetyl-LDL)
RN  - 0 (podocalyxin-like protein 1, Oryctolagus cuniculus)
RN  - 106956-32-5 (Oncostatin M)
RN  - EC 3.1.3.48 (Leukocyte Common Antigens)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Aorta/chemistry/*embryology
MH  - Biomarkers
MH  - Cell Differentiation/drug effects
MH  - Cell Lineage
MH  - Cells, Cultured
MH  - Chromosome Mapping
MH  - Coculture Techniques
MH  - Endothelium, Vascular/cytology/*embryology
MH  - Fetal Proteins/*analysis
MH  - Gestational Age
MH  - Gonads/chemistry/*embryology
MH  - Growth Substances/pharmacology
MH  - Hematopoietic Stem Cell Transplantation
MH  - Hematopoietic Stem Cells/chemistry/cytology/drug effects
MH  - Leukocyte Common Antigens/analysis
MH  - Lipoproteins, LDL/metabolism
MH  - Membrane Glycoproteins/*analysis/genetics
MH  - Mesonephros/*chemistry
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Molecular Sequence Data
MH  - Oncostatin M
MH  - Peptides/pharmacology/*physiology
MH  - Stem Cells/*chemistry/classification/drug effects
EDAT- 1999/12/11 00:00
MHDA- 1999/12/11 00:01
CRDT- 1999/12/11 00:00
PHST- 1999/12/11 00:00 [pubmed]
PHST- 1999/12/11 00:01 [medline]
PHST- 1999/12/11 00:00 [entrez]
AID - S1074-7613(00)80132-6 [pii]
AID - 10.1016/s1074-7613(00)80132-6 [doi]
PST - ppublish
SO  - Immunity. 1999 Nov;11(5):567-78. doi: 10.1016/s1074-7613(00)80132-6.