PMID- 1059088
OWN - NLM
STAT- MEDLINE
DCOM- 19760116
LR  - 20190501
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 72
IP  - 8
DP  - 1975 Aug
TI  - Thromboxanes: a new group of biologically active compounds derived from
      prostaglandin endoperoxides.
PG  - 2994-8
AB  - An unstable [t1/2 at 37 degrees = 32 +/- 2 (SD) sec] intermediate, thromboxane
      A2, was detected in the conversion of prostaglandin G2 into
      8-(1-hydroxy-3-oxopropyl)-9,12L-dihydroxy-5,10-heptadecadienoic acid (thromboxane
      B2) in platelets. The intermediate was trapped by addition of methanol, ethanol, 
      or sodium azide to suspensions of washed human platelets incubated for 30 sec
      with arachidonic acid or prostaglandin G2. The structures of the resulting
      derivatives demonstrated that the intermediate possessed an oxane ring as in
      thromboxane B2 but lacked its hemiacetal hydroxyl group. Additional experiments
      using 18O2 or [2H8]arachidonic acid in the formation of thromboxane B2 and CH3O2H
      for the trapping of thromboxane A2, together with information on the t1/2 of the 
      intermediate, indicated the presence of an oxetane structure in thromboxane A2.
      Incubation of arachidonic acid or prostaglandin G2 with washed platelets led to
      formation of an unstable factor that induced irreversible platelet aggregation
      and caused release of [14C]serotonin from platelets that had been incubated with 
      [14C]serotonin. The properties and the mode of formation of this factor indicated
      that it was identical with thromboxane A2. Furthermore, evidence is presented
      that the more unstable and major component of rabbit aorta contracting substance 
      (RCS) formed in platelets and guinea pig lung is also thromboxane A2.
FAU - Hamberg, M
AU  - Hamberg M
FAU - Svensson, J
AU  - Svensson J
FAU - Samuelsson, B
AU  - Samuelsson B
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (Arachidonic Acids)
RN  - 0 (Hydroxy Acids)
RN  - 0 (Peroxides)
RN  - 0 (Prostaglandins)
RN  - 0 (Pyrans)
RN  - 333DO1RDJY (Serotonin)
SB  - IM
MH  - Arachidonic Acids/metabolism/pharmacology
MH  - Blood Platelets/metabolism
MH  - Humans
MH  - Hydroxy Acids/*pharmacology
MH  - Mass Spectrometry
MH  - Peroxides
MH  - Platelet Aggregation/drug effects
MH  - Prostaglandins
MH  - Pyrans/*pharmacology
MH  - Serotonin/metabolism
PMC - PMC432905
EDAT- 1975/08/01 00:00
MHDA- 1975/08/01 00:01
CRDT- 1975/08/01 00:00
PHST- 1975/08/01 00:00 [pubmed]
PHST- 1975/08/01 00:01 [medline]
PHST- 1975/08/01 00:00 [entrez]
AID - 10.1073/pnas.72.8.2994 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 1975 Aug;72(8):2994-8. doi: 10.1073/pnas.72.8.2994.