PMID- 10589677
OWN - NLM
STAT- MEDLINE
DCOM- 19991228
LR  - 20190705
IS  - 0092-8674 (Print)
IS  - 0092-8674 (Linking)
VI  - 99
IP  - 5
DP  - 1999 Nov 24
TI  - Shroom, a PDZ domain-containing actin-binding protein, is required for neural
      tube morphogenesis in mice.
PG  - 485-97
AB  - Using gene trap mutagenesis, we have identified a mutation in mice that causes
      exencephaly, acrania, facial clefting, and spina bifida, all of which can be
      attributed to failed neural tube closure. This mutation is designated shroom
      (shrm) because the neural folds "mushroom" outward and do not converge at the
      dorsal midline. shrm encodes a PDZ domain protein that is involved at several
      levels in regulating aspects of cytoarchitecture. First, endogenous Shrm
      localizes to adherens junctions and the cytoskeleton. Second, ectopically
      expressed Shrm alters the subcellular distribution of F-actin. Third, Shrm
      directly binds F-actin. Finally, cytoskeletal polarity within the neuroepithelium
      is perturbed in mutant embryos. In concert, these observations suggest that Shrm 
      is a critical determinant of the cellular architecture required for proper
      neurulation.
FAU - Hildebrand, J D
AU  - Hildebrand JD
AD  - Program in Developmental Biology and Division of Basic Sciences, Fred Hutchinson 
      Cancer Research Center, Seattle, Washington 98109-1024, USA.
FAU - Soriano, P
AU  - Soriano P
LA  - eng
SI  - GENBANK/AF199421
SI  - GENBANK/AF199422
GR  - HD24875/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
RN  - 0 (Actins)
RN  - 0 (Microfilament Proteins)
RN  - 0 (Shrm protein, mouse)
SB  - IM
MH  - Actins/*metabolism
MH  - Amino Acid Sequence
MH  - Animals
MH  - Body Patterning
MH  - Cell Compartmentation
MH  - Cell Polarity/physiology
MH  - Central Nervous System/cytology/*embryology
MH  - Cytoskeleton/physiology
MH  - Epithelial Cells/physiology
MH  - Genes, Reporter
MH  - Intercellular Junctions
MH  - Mice
MH  - Mice, Mutant Strains
MH  - Microfilament Proteins/*genetics
MH  - Molecular Sequence Data
MH  - Morphogenesis
MH  - Mutation
MH  - Neural Tube Defects/*genetics
MH  - Phenotype
MH  - Protein Binding
MH  - Protein Structure, Tertiary
MH  - Sequence Homology, Amino Acid
MH  - Tissue Distribution
EDAT- 1999/12/10 00:00
MHDA- 1999/12/10 00:01
CRDT- 1999/12/10 00:00
PHST- 1999/12/10 00:00 [pubmed]
PHST- 1999/12/10 00:01 [medline]
PHST- 1999/12/10 00:00 [entrez]
AID - S0092-8674(00)81537-8 [pii]
AID - 10.1016/s0092-8674(00)81537-8 [doi]
PST - ppublish
SO  - Cell. 1999 Nov 24;99(5):485-97. doi: 10.1016/s0092-8674(00)81537-8.