PMID- 10588946
OWN - NLM
STAT- MEDLINE
DCOM- 20000210
LR  - 20120216
IS  - 0022-2275 (Print)
IS  - 0022-2275 (Linking)
VI  - 40
IP  - 12
DP  - 1999 Dec
TI  - Family of human oxysterol binding protein (OSBP) homologues. A novel member
      implicated in brain sterol metabolism.
PG  - 2204-11
AB  - Oxysterol binding protein (OSBP) is a cytosolic protein that undergoes
      ligand-induced binding to the Golgi apparatus and has been implicated in the
      regulation of cellular cholesterol metabolism. In the yeast Saccharomyces
      cerevisiae an OSBP homologue is involved in membrane trafficking through the
      Golgi complex. Prompted by the multitude of OSBP-related genes in the yeast
      genome, we carried out a search for human expressed sequence tags (ESTs)
      displaying homology to the sterol-binding domain of OSBP. This revealed a minimum
      of six novel OSBP-related proteins, designated ORP-1 to ORP-6. ORP cDNA probes
      were generated by reverse transcription-PCR from human liver mRNA, and used for
      Northern blot analysis of human tissue transcript panels. This verified that each
      of them represents a different gene product and showed that they display distinct
      tissue-specific expression patterns. The ORP-1 and -2 mRNA expression levels were
      similar to or higher than that of OSBP while the ORP-3 to -6 mRNAs were detected 
      at lower levels in specific tissues. The most abundantly expressed new gene,
      ORP-1, was transcribed at strikingly high levels in the cortical areas of human
      brain and displayed sterol-regulated expression in a cultured human neuroblastoma
      cell line. This indicates that ORP-1 may play an important role in maintaining
      the sterol balance in cells of the central nervous system. Together with OSBP,
      the identified gene products constitute a novel human protein family that may
      provide a link between organellar sterol status and membrane dynamics.
FAU - Laitinen, S
AU  - Laitinen S
AD  - Department of Biochemistry, National Public Health Institute, Mannerheimintie
      166, 00300, Helsinki, Finland.
FAU - Olkkonen, V M
AU  - Olkkonen VM
FAU - Ehnholm, C
AU  - Ehnholm C
FAU - Ikonen, E
AU  - Ikonen E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Lipid Res
JT  - Journal of lipid research
JID - 0376606
RN  - 0 (DNA, Complementary)
RN  - 0 (Protein Isoforms)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Steroid)
RN  - 0 (Sterols)
RN  - 0 (oxysterol binding protein)
SB  - IM
MH  - Binding Sites/genetics
MH  - Blotting, Northern
MH  - Brain Chemistry
MH  - DNA, Complementary/biosynthesis
MH  - Databases, Factual
MH  - Gene Expression
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - Liver/cytology/metabolism
MH  - Neuroblastoma/genetics
MH  - Protein Isoforms/*genetics
MH  - RNA, Messenger/analysis/genetics
MH  - Receptors, Steroid/*chemistry/*genetics
MH  - Sequence Homology, Nucleic Acid
MH  - Sterols/pharmacology
MH  - Tumor Cells, Cultured/metabolism
EDAT- 1999/12/10 00:00
MHDA- 1999/12/10 00:01
CRDT- 1999/12/10 00:00
PHST- 1999/12/10 00:00 [pubmed]
PHST- 1999/12/10 00:01 [medline]
PHST- 1999/12/10 00:00 [entrez]
PST - ppublish
SO  - J Lipid Res. 1999 Dec;40(12):2204-11.