PMID- 10588641
OWN - NLM
STAT- MEDLINE
DCOM- 20000214
LR  - 20181113
IS  - 1059-1524 (Print)
IS  - 1059-1524 (Linking)
VI  - 10
IP  - 12
DP  - 1999 Dec
TI  - Phosphorylation of SNAP-23 by the novel kinase SNAK regulates t-SNARE complex
      assembly.
PG  - 4033-41
AB  - The docking and fusion of cargo-containing vesicles with target membranes of
      eukaryotic cells is mediated by the interaction of SNARE proteins present on both
      vesicle and target membranes. In many cases, the target membrane SNARE, or
      t-SNARE, exists as a complex of syntaxin with a member of the SNAP-25 family of
      palmitoylated proteins. We have identified a novel human kinase SNAK (SNARE
      kinase) that specifically phosphorylates the nonneuronal t-SNARE SNAP-23 in vivo.
      Interestingly, only SNAP-23 that is not assembled into t-SNARE complexes is
      phosphorylated by SNAK, and phosphorylated SNAP-23 resides exclusively in the
      cytosol. Coexpression with SNAK significantly enhances the stability of
      unassembled SNAP-23, and as a consequence, the assembly of newly synthesized
      SNAP-23 with syntaxin is augmented. These data demonstrate that phosphorylation
      of SNAP-23 by SNAK enhances the kinetics of t-SNARE assembly in vivo.
FAU - Cabaniols, J P
AU  - Cabaniols JP
AD  - Experimental Immunology Branch, National Cancer Institute, National Institutes of
      Health, Bethesda, Maryland 20892, USA.
FAU - Ravichandran, V
AU  - Ravichandran V
FAU - Roche, P A
AU  - Roche PA
LA  - eng
SI  - GENBANK/AF246219
PT  - Journal Article
PL  - United States
TA  - Mol Biol Cell
JT  - Molecular biology of the cell
JID - 9201390
RN  - 0 (Carrier Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Qa-SNARE Proteins)
RN  - 0 (Qb-SNARE Proteins)
RN  - 0 (Qc-SNARE Proteins)
RN  - 0 (SNAP23 protein, human)
RN  - 0 (SNARE Proteins)
RN  - 0 (Vesicular Transport Proteins)
RN  - EC 2.7.11.1 (Protein-Serine-Threonine Kinases)
SB  - IM
MH  - Carrier Proteins/*metabolism
MH  - HeLa Cells
MH  - Humans
MH  - Membrane Proteins/*metabolism
MH  - Phosphorylation
MH  - Protein Processing, Post-Translational
MH  - Protein-Serine-Threonine Kinases/*genetics/isolation & purification/metabolism
MH  - Qa-SNARE Proteins
MH  - Qb-SNARE Proteins
MH  - Qc-SNARE Proteins
MH  - SNARE Proteins
MH  - *Vesicular Transport Proteins
PMC - PMC25741
EDAT- 1999/12/10 09:00
MHDA- 2000/02/19 09:00
CRDT- 1999/12/10 09:00
PHST- 1999/12/10 09:00 [pubmed]
PHST- 2000/02/19 09:00 [medline]
PHST- 1999/12/10 09:00 [entrez]
AID - 10.1091/mbc.10.12.4033 [doi]
PST - ppublish
SO  - Mol Biol Cell. 1999 Dec;10(12):4033-41. doi: 10.1091/mbc.10.12.4033.